cDNA cloning and sequence analysis of Lys-49 phospholipase A2 from Agkistrodon acutus

被引：10

作者：

Fan, CY ^{[1
]}

Qian, YC ^{[1
]}

Yang, SL ^{[1
]}

Gong, Y ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai 200233, Peoples R China

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1999年 / 15卷 / 01期

关键词：

phospholipase A(2); Agkistrodon acutus; cDNA sequence analysis; myotoxin;

D O I：

10.1016/S1050-3862(98)00033-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Total RNA was extracted from venom glands of Agkistrodon acutus. The cDNA encoding Lys-49 phospholipase A(2) (PLA(2)) was amplified by reverse transcriptional polymerase chain reaction (RT-PCR). The cDNA was cloned into the pGEMT-vector and sequenced. The open reading frame (ORF) of Lys-49 PLA(2) consists of 414 bp encoding 138 amino acids, which includes a signal peptide of 16 amino acids and a matured peptide of 122 amino acids. It shows 76% identity in amino acids with another reported Lys-49 PLA(2). Because residue 49 in mature peptide is Lysine, it probably possesses myotoxicity. These results indicate there are at least two kinds of myotoxin in the venom of A. acutus. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：15 / 18

页数：4

共 17 条

[1]

[Anonymous], [No title captured]

[2] Phospholipase A(2) - A structural review [J].

Arni, RK ;

Ward, RJ .

TOXICON, 1996, 34 (08) :827-841

[3] CLONING AND SEQUENCING OF CDNAS ENCODING THE 2 SUBUNITS OF CROTOXIN [J].

BOUCHIER, C ;

DUCANCEL, F ;

GUIGNERYFRELAT, G ;

BON, C ;

BOULAIN, JC ;

MENEZ, A .

NUCLEIC ACIDS RESEARCH, 1988, 16 (18) :9050-9050

[4] BOTHROPSTOXIN-I - AMINO-ACID-SEQUENCE AND FUNCTION [J].

CINTRA, ACO ;

MARANGONI, S ;

OLIVEIRA, B ;

GIGLIO, JR .

JOURNAL OF PROTEIN CHEMISTRY, 1993, 12 (01) :57-64

[5] Sequence analysis of LYS49 phospholipase A(2) myotoxins: A highly conserved class of proteins [J].

DeAraujo, HSS ;

White, SP ;

Ownby, CL .

TOXICON, 1996, 34 (11-12) :1237-1242

[6] CDNA cloning and sequence analysis of a lysine-49 phospholipase A(2) myotoxin from Agkistrodon contortrix laticinctus snake venom [J].

deAraujo, HSS ;

White, SP ;

Ownby, CL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1996, 326 (01) :21-30

[7]

DENNIS EA, 1994, J BIOL CHEM, V269, P13057

[8] MYOTOXIN-II FROM BOTHROPS-ASPER (TERCIOPELO) VENOM IS A LYSINE-49 PHOSPHOLIPASE-A2 [J].

FRANCIS, B ;

GUTIERREZ, JM ;

LOMONTE, B ;

KAISER, II .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 284 (02) :352-359

[9] GENOMIC SEQUENCES ENCODING THE ACIDIC AND BASIC SUBUNITS OF MOJAVE TOXIN - UNUSUALLY HIGH SEQUENCE IDENTITY OF NONCODING REGIONS [J].

JOHN, TR ;

SMITH, LA ;

KAISER, II .

GENE, 1994, 139 (02) :229-234

[10] PURIFICATION AND AMINO-ACID-SEQUENCE OF BASIC PROTEIN-II, A LYSINE-49-PHOSPHOLIPASE-A2 WITH LOW ACTIVITY, FROM TRIMERESURUS-FLAVOVIRIDIS VENOM [J].

LIU, SY ;

YOSHIZUMI, K ;

ODA, N ;

OHNO, M ;

TOKUNAGA, F ;

IWANAGA, S ;

KIHARA, H .

JOURNAL OF BIOCHEMISTRY, 1990, 107 (03) :400-408

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