Induction of cytotoxicity of Pelagia noctiluca venom causes reactive oxygen species generation, lipid peroxydation induction and DNA damage in human colon cancer cells

被引：31

作者：

Ayed, Yosra ^{[1
]}

Boussabbeh, Manel ^{[1
]}

Zakhama, Wiem ^{[1
]}

Bouaziz, Chayma ^{[1
]}

Abid, Salwa ^{[1
]}

Bacha, Hassen ^{[1
,2
]}

机构：

[1] Fac Dent, Lab Res Biol Compatible Cpds, Monastir 5019, Tunisia

[2] Univ Jendouba, Jendouba 8100, Tunisia

来源：

LIPIDS IN HEALTH AND DISEASE | 2011年 / 10卷

关键词：

Pelagia noctiluca; Jellyfish; Venom; Cytotoxicity; Oxidative stress; DNA fragmentation; SEA-ANEMONE; EQUINATOXIN-II; CRUDE VENOM; MAMMALIAN-CELLS; JELLYFISH; CNIDARIA; TOXICITY; ASSAY; MALONALDEHYDE; GENOTOXICITY;

D O I：

10.1186/1476-511X-10-232

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Background: The long-lasting and abundant blooming of Pelagia noctiluca in Tunisian coastal waters compromises both touristic and fishing activities and causes substantial economic losses. Determining their molecular mode of action is, important in order to limit or prevent the subsequent damages. Thus, the aim of the present study was to investigate the propensity of Pelagia noctiluca venom to cause oxidative damage in HCT 116 cells and its associated genotoxic effects. Results: Our results indicated an overproduction of ROS, an induction of catalase activity and an increase of MDA generation. We looked for DNA fragmentation by means of the comet assay. Results indicated that venom of Pelagia noctiluca induced DNA fragmentation. SDS-PAGE analysis of Pelagia noctiluca venom revealed at least 15 protein bands of molecular weights ranging from 4 to 120 kDa. Conclusion: Oxidative damage may be an initiating event and contributes, in part, to the mechanism of toxicity of Pelagia noctiluca venom.

引用

页数：10

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