Lymphangiogenesis quantification using quantitative PCR and breast cancer as a model

被引：49

作者：

Cunnick, GH ^{[1
]}

Jiang, WG ^{[1
]}

Gomez, KF ^{[1
]}

Mansel, RE ^{[1
]}

机构：

[1] Univ Wales Coll Cardiff, Coll Med, Dept Surg, Metastasis Res Grp, Cardiff CF14 4XN, S Glam, Wales

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 288卷 / 04期

关键词：

lymphangiogenesis; LYVE-1; real-time quantitative polymerase chain reaction; breast cancer;

D O I：

10.1006/bbrc.2001.5869

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The detection of lymphangiogenesis (formation of new lymphatics) has previously been difficult to measure, primarily due to the lack of specific markers for lymphatic endothelium. Using conventional PCR (polymerase chain reaction), DNA sequencing, plasmid synthesis, and real-time quantitative PCR (RTQPCR), we report a new approach to enable the measurement of lymphangiogenesis using LYVE-1, a novel, specific lymphatic marker in breast cancer tissue. By using a Scorpion-based probe system with the RTQPCR analyser, a highly sensitive and specific detection and quantitation of LYVE-1 was possible. It was found that lymphangiogenesis occurred in all breast specimens and that higher levels were found in tumours which had spread to the lymph nodes. (C) 2001 Academic Press.

引用

页码：1043 / 1046

页数：4

共 7 条

[1] LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan [J].