(E)-β-ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon:: Function and expression of three terpene synthase genes of a new terpene synthase subfamily

被引:371
作者
Dudareva, N [1 ]
Martin, D
Kish, CM
Kolosova, N
Gorenstein, N
Fäldt, J
Miller, B
Bohlmann, J
机构
[1] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA
[2] Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1Z3, Canada
[3] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z3, Canada
[4] Univ British Columbia, Dept Forest Sci, Vancouver, BC V6T 1Z3, Canada
关键词
D O I
10.1105/tpc.011015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Snapdragon flowers emit two monoterpene olefins, myrcene and (E)-beta-ocimene, derived from geranyl diphosphate, in addition to a major phenylpropanoid floral scent component, methylbenzoate. Emission of these monoterpenes is regulated developmentally and follows diurnal rhythms controlled by a circadian clock. Using a functional genomics approach, we have isolated and characterized three closely related cDNAs from a snapdragon petal-specific library that encode two myrcene synthases (ama1e20 and ama0c15) and an (E)-beta-ocimene synthase (ama0a23). Although the two myrcene synthases are almost identical (98%), except for the N-terminal 13 amino acids, and are catalytically active, yielding a single monoterpene product, myrcene, only ama0c15 is expressed at a high level in flowers and contributes to floral myrcene emission. (E)-beta-Ocimene synthase is highly similar to snapdragon myrcene synthases (92% amino acid identity) and produces predominantly (E)-beta-ocimene (97% of total monoterpene olefin product) with small amounts of (Z)-beta-ocimene and myrcene. These newly isolated snapdragon monoterpene synthases, together with Arabidopsis AtTPS14 (At1g61680), define a new subfamily of the terpene synthase (TPS) family designated the Tps-g group. Members of this new Tps-g group lack the RRX8W motif, which is a characteristic feature of the Tps-d and Tps-b monoterpene synthases, suggesting that the reaction mechanism of Tps-g monoterpene synthase product formation does not proceed via an RR-dependent isomerization of geranyl diphosphate to 3S-linalyl diphosphate, as shown previously for limonene cyclase. Analyses of tissue-specific, developmental, and rhythmic expression of these monoterpene synthase genes in snapdragon flowers revealed coordinated regulation of phenylpropanoid and isoprenoid scent production.
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页码:1227 / 1241
页数:15
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