Human POGZ modulates dissociation of HP1α from mitotic chromosome arms through Aurora B activation

被引:202
作者
Nozawa, Ryu-Suke [1 ]
Nagao, Koji [1 ,2 ]
Masuda, Hiro-Taka [1 ]
Iwasaki, Osamu [1 ]
Hirota, Toru [3 ]
Nozaki, Naohito [4 ]
Kimura, Hiroshi [5 ]
Obuse, Chikashi [1 ]
机构
[1] Hokkaido Univ, Grad Sch Life Sci, Sapporo, Hokkaido 0010021, Japan
[2] Okinawa Inst Sci & Technol, Initial Res Project, Okinawa 9042234, Japan
[3] Japanese Fdn Canc Res, Inst Canc, Tokyo 1358550, Japan
[4] Kanagawa Dent Coll, Yokosuka, Kanagawa 2388580, Japan
[5] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan
基金
日本学术振兴会;
关键词
HP1; PROTEINS; PERICENTRIC HETEROCHROMATIN; KINETOCHORE PROTEIN; OUTER KINETOCHORE; HUMAN-CELLS; COMPLEX; METHYLATION; DOMAIN; GENE; CENTROMERE;
D O I
10.1038/ncb2075
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Heterochromatin protein 1 (HP1) has an essential role in heterochromatin formation and mitotic progression through its interaction with various proteins. We have identified a unique HP1 alpha-binding protein, POGZ (pogo transposable element-derived protein with zinc finger domain), using an advanced proteomics approach. Proteins generally interact with HP1 through a PxVxL (where x is any amino-acid residue) motif; however, POGZ was found to bind to HP1 alpha through a zinc-finger-like motif. Binding by POGZ, mediated through its zinc-finger-like motif, competed with PxVxL proteins and destabilized the HP1 alpha-chromatin interaction. Depletion experiments confirmed that the POGZ HP1-binding domain is essential for normal mitotic progression and dissociation of HP1 alpha from mitotic chromosome arms. Furthermore, POGZ is required for the correct activation and dissociation of Aurora B kinase from chromosome arms during M phase. These results reveal POGZ as an essential protein that links HP1 alpha dissociation with Aurora B kinase activation during mitosis.
引用
收藏
页码:719 / U212
页数:35
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