Caged RNA: photo-control of a ribozyme reaction

被引：113

作者：

Chaulk, SG ^{[1
]}

MacMillan, AM ^{[1
]}

机构：

[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3HC, Canada

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 13期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/26.13.3173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the first photo-chemical control of a ribozyme reaction by the site-specific modification of the 2'-hydroxyl nucleophile in the hammerhead system with a caging functionality. Rapid laser photolysis of the O-(2-nitrobenzyl) caging group initiates an efficient and accurate hammerhead-catalyzed cleavage of substrate RNA under native conditions. RNAs in which reactive functionalities or recognition elements are caged in this manner will be useful tools to probe RNA reactivity and dynamics.

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页码：3173 / 3178

页数：6

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