Transcriptional program controlled by the floral homeotic gene AGAMOUS during early organogenesis

被引:275
作者
Gómez-Mena, C
de Folter, S
Costa, MMR
Angenent, GC
Sablowski, R [1 ]
机构
[1] John Innes Ctr Plant Sci Res, Dept Cell & Dev Biol, Norwich NR4 7UH, Norfolk, England
[2] Plant Res Int, Business Unit Biosci, NL-6700 AA Wageningen, Netherlands
来源
DEVELOPMENT | 2005年 / 132卷 / 03期
基金
英国生物技术与生命科学研究理事会;
关键词
homeotic genes; floral development; transcription; AGAMOUS;
D O I
10.1242/dev.01600
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Floral organs, whose identity is determined by specific combinations of homeotic genes, originate from a group of undifferentiated cells called the floral meristem. In Arabidopsis, the homeotic gene AGAMOUS (AG) terminates meristem activity and promotes development of stamens and carpels. To understand the program of gene expression activated by AG, we followed genome-wide expression during early stamen and carpel development. The AG target genes included most genes for which mutant screens revealed a function downstream of AG. Novel targets were validated by in situ hybridisation and binding to AG in vitro and in vivo. Transcription factors formed a large fraction of AG targets, suggesting that during early organogenesis, much of the genetic program is concerned with elaborating gene expression patterns. The results also suggest that AG and other homeotic proteins with which it interacts (SEPALLATA3, APETALA3, PISTILLATA) are coordinately regulated in a positive-feedback loop to maintain their own expression, and that AG activates biosynthesis of gibberellin, which has been proposed to promote the shift from meristem identity to differentiation.
引用
收藏
页码:429 / 438
页数:10
相关论文
共 70 条
[1]   Molecular characterization of the CER1 gene of arabidopsis involved in epicuticular wax biosynthesis and pollen fertility [J].
Aarts, MGM ;
Keijzer, CJ ;
Stiekema, WJ ;
Pereira, A .
PLANT CELL, 1995, 7 (12) :2115-2127
[2]  
Alvarez J, 1999, DEVELOPMENT, V126, P2377
[3]   Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter [J].
Blázquez, MA ;
Green, R ;
Nilsson, O ;
Sussman, MR ;
Weigel, D .
PLANT CELL, 1998, 10 (05) :791-800
[4]  
Bowman J L, 1991, Symp Soc Exp Biol, V45, P89
[5]  
Bowman JL, 1999, DEVELOPMENT, V126, P2387
[6]   A genomic regulatory network for development [J].
Davidson, EH ;
Rast, JP ;
Oliveri, P ;
Ransick, A ;
Calestani, C ;
Yuh, CH ;
Minokawa, T ;
Amore, G ;
Hinman, V ;
Arenas-Mena, C ;
Otim, O ;
Brown, CT ;
Livi, CB ;
Lee, PY ;
Revilla, R ;
Rust, AG ;
Pan, ZJ ;
Schilstra, MJ ;
Clarke, PJC ;
Arnone, MI ;
Rowen, L ;
Cameron, RA ;
McClay, DR ;
Hood, L ;
Bolouri, H .
SCIENCE, 2002, 295 (5560) :1669-1678
[7]   The role of JAGGED in shaping lateral organs [J].
Dinneny, JR ;
Yadegari, R ;
Fischer, RL ;
Yanofsky, MF ;
Weigel, D .
DEVELOPMENT, 2004, 131 (05) :1101-1110
[8]   Distinct mechanisms promote polarity establishment in carpels of Arabidopsis [J].
Eshed, Y ;
Baum, SF ;
Bowman, JL .
CELL, 1999, 99 (02) :199-209
[9]  
Ferrándiz C, 2000, DEVELOPMENT, V127, P725
[10]   Conservation and diversity in flower land [J].
Ferrario, S ;
Immink, RG ;
Angenent, GC .
CURRENT OPINION IN PLANT BIOLOGY, 2004, 7 (01) :84-91