Immunoreactive orexin-A in human plasma

被引：88

作者：

Arihara, Z

Takahashi, K ^{[1
]}

Murakami, O

Totsune, K

Sone, M

Satoh, F

Ito, S

Mouri, T

机构：

[1] Tohoku Univ, Sch Med, Dept Mol Biol & Appl Physiol, Aoba Ku, Sendai, Miyagi 9808575, Japan

[2] Tohoku Univ, Sch Med, Dept Internal Med 2, Sendai, Miyagi 9808574, Japan

[3] Natl Iwate Hosp, Dept Internal Med, Ichinoseki, Iwate 0210015, Japan

[4] Mouri Clin, Div Neuroendocrinol, Sendai, Miyagi 9811224, Japan

来源：

PEPTIDES | 2001年 / 22卷 / 01期

关键词：

orexin-A; plasma; diurnal variation; radioimmunoassay;

D O I：

10.1016/S0196-9781(00)00369-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Orexin-A and orexin-B are newly discovered neuropeptides which art: implicated in feeding behavior and arousal state. We studied immunoreactive(IR)-orexin-A concentrations in human plasma by radioimmunoassay. IR-orexin-A concentrations in plasma obtained from 17 healthy subjects in the morning were 1.94 +/- 0.24 pmol/liter (mean ir SEM). IR-orexin-A levels in the plasma obtained at night were not significantly different from those obtained in the morning in 9 female subjects. The HPLC analysis of the plasma extract showed two immunoreactive peaks; one peak eluting in an identical position to synthetic orexin-A, and one eluting earlier. This study has shown for the first time the presence of orexin-A in human plasma. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：139 / 142

页数：4

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