Role of superoxide anion on the proliferation and c-Ha-ras or p53 expression in prostate cancer cell line PC3

被引:9
作者
Wang, ZP
Zheng, RL
Fu, SJ
Chen, YR [1 ]
Duan, GL
Qin, DS
Liu, GD
机构
[1] Lanzhou Med Coll, Affiliated Hosp 2, Inst Urol, Lanzhou 730030, Peoples R China
[2] Lanzhou Univ, Dept Biol, Lanzhou 730000, Peoples R China
来源
UROLOGICAL RESEARCH | 1998年 / 26卷 / 05期
关键词
superoxide anion; proliferation; gene expression; p53; c-Ha-ras; prostate cancer cell;
D O I
10.1007/s002400050068
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
The purpose of this study was to investigate the role of superoxide anion (O-2(-.)) in the regulation of p53 or c-Ha-ras expression and proliferation in the prostate cancer cell line PC3. Cell proliferation was tested by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay in the presence of O-2(-.), basic fibroblast growth factor (bFGF) or their combination. p53 or C-Ha-ras expression in the cells treated with O-2(-.) was assayed by fluorescence in situ hybridization (FISH). The proliferation was significantly inhibited by O-2(-.) in a concentration-dependent manner ranging from 9 to 36 mu mol/l nicotinamide adenine dinucleotid (NADH) combined with 2-8 mu mol/l N-methylphenazonium methyl sulfate (PMS). Enhancement of proliferation by 2 ng/ml bFGF was significantly inhibited by;. Although O-2(-.) was not able to alter c-Ha-ras gene expression, O-2(-.) at the concentrations of 18 mu mol/l NADH and 4 mu mol/l PMS upregulated the expression of p53. O-2(-.) may modulate proliferation and gene expression in PC3 cells.
引用
收藏
页码:349 / 353
页数:5
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