Targeted disruption of the mouse phospholipase C β3 gene results in early embryonic lethality

被引：51

作者：

Wang, S

Gebre-Medhin, S

Betsholtz, C

Stålberg, P

Zhou, YH

Larsson, C

Weber, G

Feinstein, R

Öberg, K

Gobl, A

Skogseid, B ^{[1
]}

机构：

[1] Univ Uppsala Hosp, Dept Internal Med, S-75185 Uppsala, Sweden

[2] Gothenburg Univ, Dept Med Biochem, S-41390 Gothenburg, Sweden

[3] Karolinska Hosp, Dept Clin Genet, S-10401 Stockholm, Sweden

[4] Natl Vet Inst, Dept Pathol, S-75007 Uppsala, Sweden

来源：

FEBS LETTERS | 1998年 / 441卷 / 02期

关键词：

phospholipase C beta 3; signal transduction; targeted disruption; early embryonic development; preimplantational lethality;

D O I：

10.1016/S0014-5793(98)01518-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to investigate the biological function of phosphatidylinositol-specific phospholipase C (PLC) we generated mutant mice by gene targeting. Homozygous inactivation of PLC beta(3) is lethal at embryonic day 2.5. These mutants show poor embryonic organization as well as reduced numbers of cells, Identical phenotypes were recorded in homozygous mutants generated from two independently targeted embryonic stem cell clones. Heterozygous mutant mice, however, are viable and fertile for at least two generations. We also showed that mouse PLC beta(3) is expressed in unfertilized eggs, 3-cell and egg cylinder stages of embryos. In conclusion, these results indicate that PLC beta(3) expression is essential for early mouse embryonic development. (C) 1998 Federation of European Biochemical Societies.

引用

页码：261 / 265

页数：5

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