Prevalence and genetic variability of Arcobacter species in mechanically separated turkey

被引:45
作者
Manke, TR
Wesley, IV
Dickson, JS [1 ]
Harmon, KM
机构
[1] Iowa State Univ, Dept Microbiol Immunol & Prevent Med, Ames, IA 50011 USA
[2] USDA ARS, Natl Anim Dis Ctr, Enter Dis & Food Safety Res Unit, Ames, IA 50010 USA
关键词
D O I
10.4315/0362-028X-61.12.1623
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A survey for Arcobacter spp, and Arcobacter butzleri in mechanically separated turkey was conducted during the winter of 1995 and summer and fall of 1996. Arcobacter spp. and A. butzleri were identified by polymerase chain reaction and species-specific oligonucleotide probes. Arcobacter spp. were isolated from 77% (303 out of 395) of the mechanically separated turkey samples with 74% (223 out of 303) of these samples positive for A. butzleri. Of the 121 A. butzleri isolates tested, 86 different patterns were evident following amplification of enterobacterial repetitive intergenic consensus sequences. The extent of generic polymorphism indicated multiple sources of contamination.
引用
收藏
页码:1623 / 1628
页数:6
相关论文
共 35 条
[1]   ARCOBACTER-(CAMPYLOBACTER)-BUTZLERI-ASSOCIATED DIARRHEAL ILLNESS IN A NONHUMAN PRIMATE POPULATION [J].
ANDERSON, KF ;
KIEHLBAUCH, JA ;
ANDERSON, DC ;
MCCLURE, HM ;
WACHSMUTH, IK .
INFECTION AND IMMUNITY, 1993, 61 (05) :2220-2223
[2]   The prevalence of Campylobacters and Arcobacters in broiler chickens [J].
Atabay, HI ;
Corry, JEL .
JOURNAL OF APPLIED MICROBIOLOGY, 1997, 83 (05) :619-626
[3]   A variable 23S rDNA region is a useful discriminating target for genus-specific and species-specific PCR amplification in Arcobacter species [J].
Bastyns, K ;
Cartuyvels, D ;
Chapelle, S ;
Vandamme, P ;
Goossens, H ;
DeWachter, R .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1995, 18 (03) :353-356
[4]   Detection of Arcobacter spp in ground pork by modified plating methods [J].
Collins, CI ;
Wesley, IV ;
Murano, EA .
JOURNAL OF FOOD PROTECTION, 1996, 59 (05) :448-452
[5]  
deBoer E, 1996, LETT APPL MICROBIOL, V23, P64
[6]  
DHAMABUTRA N, 1992, J MED ASSOC THAILAND, V75, P3350
[7]  
ELLINGHAUSEN HC, 1965, AM J VET RES, V26, P45
[8]   ISOLATION OF SPIRILLUM-LIKE ORGANISMS FROM PIG FETUSES [J].
ELLIS, WA ;
NEILL, SD ;
OBRIEN, JJ ;
HANNA, J .
VETERINARY RECORD, 1978, 102 (05) :106-106
[9]   ISOLATION OF SPIRILLUM-VIBRIO-LIKE ORGANISMS FROM BOVINE FETUSES [J].
ELLIS, WA ;
NEILL, SD ;
OBRIEN, JJ ;
FERGUSON, HW ;
HANNA, J .
VETERINARY RECORD, 1977, 100 (21) :451-452
[10]  
Festy B., 1993, ACTA GASTROENTEROL B, V35