Identification of actin as quercetin-binding protein:: An approach to identify target molecules for specific ligands

被引：25

作者：

Böhl, M

Czupalla, C

Tokalov, SV

Hoflack, B

Gutzeit, HO ^{[1
]}

机构：

[1] Dresden Univ Technol, Inst Zool, D-01062 Dresden, Germany

[2] Dresden Univ Technol, Ctr Biotechnol, D-01307 Dresden, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 346卷 / 02期

关键词：

quercetin; actin; fluorescence quenching; target identification;

D O I：

10.1016/j.ab.2005.08.037

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The biological effect of flavonoids is commonly studied by assaying the activity of a protein of interest. Taking a reverse approach, we identified target proteins of the widely studied flavonol quercetin by exploiting the altered spectroscopic properties of target proteins and ligands on their molecular interaction. Nuclear extracts of human leukemia cells were fractionated by column chromatography and assayed for their ability to alter the fluorescence emission spectra, and finally the proteins present in fractions of interest were identified by mass spectrometry. Among the identified proteins, actin was shown to be a quercetin-binding nuclear protein. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：295 / 299

页数：5

共 15 条

[1] Identification and characterization of the autophosphorylation sites of phosphoinositide 3-kinase isoforms β and γ [J].