Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography multi-angle laser light scattering

被引：69

作者：

Jumel, K ^{[1
]}

Fiebrig, I ^{[1
]}

Harding, SE ^{[1
]}

机构：

[1] UNIV NOTTINGHAM, DEPT PHARMACEUT SCI, NOTTINGHAM NG7 2RD, ENGLAND

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 1996年 / 18卷 / 1-2期

关键词：

mucus glycoproteins; size exclusion chromatography multi angle laser light scattering; molar mass determination;

D O I：

10.1016/0141-8130(95)01071-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The weight average molar masses and molar mass distributions of two commercial pig gastric mucins and two fresh mucin preparations were determined by the technique of size exclusion chromatography coupled on-line to multi angle laser light scattering (SEC/MALLS). Both commercial samples exhibited much lower molar mass averages than the freshly prepared material and contained more impurities. On the other hand: a fresh mucin preparation examined after being stored frozen for 18 months revealed a slight increase in molar mass.

引用

页码：133 / 139

页数：7

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