Association of an acylated model peptide with DPPC-DPPS lipid membranes

被引：6

作者：

Pedersen, TB

Sabra, MC

Frokjaer, S

Mouritsen, OG

Jorgensen, K

机构：

[1] Tech Univ Denmark, Dept Chem, DK-2800 Lyngby, Denmark

[2] Royal Danish Sch Pharm, Dept Pharmaceut, DK-2100 Copenhagen, Denmark

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2001年 / 214卷 / 1-2期

关键词：

acylated peptide; peptide-membrane association; nagatively charged membranes; fluorescence resonance energy transfer;

D O I：

10.1016/S0378-5173(00)00636-0

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The interaction between a small positively charged peptide with a N-terminally linked acyl chain and dipalmitoylphosphatidylcholine-dipalmitoylphosphatidylserine (DPPC-DPPS) lipid membranes has been studied by means of fluorescence resonance energy transfer. Two different lipid compositions were used: a neutral membrane (100 mol% DPPC), and a negatively charged membrane (30 mol% DPPS in DPPC). The fluorescence resonance energy transfer results reveal that the peptide associates with both types of membranes. Furthermore, it is found that the slope of the titration curve for the negatively charged membranes is much steeper than that for the neutral membranes. This indicates a higher binding affinity of the acylated peptide towards negatively charged lipid membranes as compared with neutral lipid membranes. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：77 / 81

页数：5

共 15 条

[1] STABILITY OF ACYL DERIVATIVES OF INSULIN IN THE SMALL-INTESTINE - RELATIVE IMPORTANCE OF INSULIN ASSOCIATION CHARACTERISTICS IN AQUEOUS-SOLUTION [J].