Analysis of PU.1/ICSBP (IRF-8) complex formation with various PU.1 mutants:: molecular cloning of rat lcsbp (Irf-8) cDNA

被引：8

作者：

Nakano, N

Nishiyama, C

Masuoka, N

Nishiyama, M

Yamane, H

Okumura, K

Ogawa, H

机构：

[1] Juntendo Univ, Sch Med, Atopy Allergy Res Ctr, Bunkyo Ku, Tokyo 1138421, Japan

[2] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan

来源：

IMMUNOGENETICS | 2005年 / 56卷 / 12期

关键词：

cDNA; ICSBP; PU.1; transcription factor;

D O I：

10.1007/s00251-004-0754-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We isolated cDNA encoding a full-length Interferon (IFN) consensus sequence binding protein [Icsbp; also called IFN regulatory factor-8 (Irf-8)] from rat and analyzed interaction between ICSBP and PU.1, an Ets-family transcription factor regulating hematopoietic cell-specific promoters. Electrophoretic mobility shift assay indicated that PU.1 with deletion of the PEST domain could not bind to ICSBP, although loss of the PEST domain had no effect on DNA-binding ability of PU.1 itself. An amino-acid replacement of Ser147 by Ala of the PEST domain of PU.1 did not affect DNA-binding ability of PU.1 to form a binary complex, PU.1/DNA, but clearly decreased the ternary complex formation of PU.1/ICSBP/DNA. Phosphatase treatment of the ternary complex markedly decreased PU.1/ICSBP/DNA-complex formation. These results indicated that Ser147 of PU.1 is definitively required for forming a complex with ICSBP and phosphorylation of PU.1 and/or ICSBP is critical for formation of the ternary complex.

引用

收藏

页码：871 / 877

页数：7

相关论文

共 15 条

[1] CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-KB and stress-activated protein kinase/c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-KB-inducing kinase [J].

Akiba, H ;

Nakano, H ;

Nishinaka, S ;

Shindo, M ;

Kobata, T ;

Atsuta, M ;

Morimoto, C ;

Ware, CF ;

Malinin, NL ;

Wallach, D ;

Yagita, H ;

Okumura, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (21) :13353-13358

[2] Regulation of B lymphocyte and macrophage development by graded expression of PU.1 [J].

DeKoter, RP ;

Singh, H .

SCIENCE, 2000, 288 (5470) :1439-1441

[3] AN INTERFERON GAMMA-REGULATED PROTEIN THAT BINDS THE INTERFERON-INDUCIBLE ENHANCER ELEMENT OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I GENES [J].

DRIGGERS, PH ;

ENNIST, DL ;

GLEASON, SL ;

MAK, WH ;

MARKS, MS ;

LEVI, BZ ;

FLANAGAN, JR ;

APPELLA, E ;

OZATO, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (10) :3743-3747

[4] PIP, A NOVEL IRF FAMILY MEMBER, IS A LYMPHOID-SPECIFIC, PU.1-DEPENDENT TRANSCRIPTIONAL ACTIVATOR [J].

EISENBEIS, CF ;

SINGH, H ;

STORB, U .

GENES & DEVELOPMENT, 1995, 9 (11) :1377-1387

[5] A novel-66T/C polymorphism in FcεRI α-chain promoter affecting the transcription activity:: Possible relationship to allergic diseases [J].

Hasegawa, M ;

Nishiyama, C ;

Nishiyama, M ;

Akizawa, Y ;

Mitsuishi, K ;

Ito, T ;

Kawada, H ;

Furukawa, S ;

Ra, C ;

Okumura, K ;

Ogawa, H .

JOURNAL OF IMMUNOLOGY, 2003, 171 (04) :1927-1933

[6] Reciprocal roles for CCAAT/enhancer binding protein (C/EBP) and PU.1 transcription factors in Langerhans cell commitment [J].

Iwama, A ;

Osawa, M ;

Hirasawa, R ;

Uchiyama, N ;

Kaneko, S ;

Onodera, M ;

Shibuya, K ;

Shibuya, A ;

Vinson, C ;

Tenen, DG ;

Nakauchi, H .

JOURNAL OF EXPERIMENTAL MEDICINE, 2002, 195 (05) :547-558

[7] PU.1/interferon regulatory factor interactions -: Mechanisms of transcriptional regulation [J].

Marecki, S ;

Fenton, MJ .

CELL BIOCHEMISTRY AND BIOPHYSICS, 2000, 33 (02) :127-148

[8] IFN-stimulated gene 15 is synergistically activated through interactions between the myelocyte/lymphocyte-specific transcription factors, PU.1, IFN regulatory factor-8/IFN consensus sequence binding protein, and IFN regulatory factor-4: Characterization of a new subtype of IFN-stimulated response element [J].

Meraro, D ;

Gleit-Kielmanowicz, M ;

Hauser, H ;

Levi, BZ .

JOURNAL OF IMMUNOLOGY, 2002, 168 (12) :6224-6231

[9]

Meraro D, 1999, J IMMUNOL, V163, P6468

[10] Evidence against requirement of Ser41 and Ser45 for function of PU.1 - Molecular cloning of rat PU.1 [J].

Nishiyama, C ;

Masuoka, N ;

Nishiyama, M ;

Ito, T ;

Yamane, H ;

Okumura, K ;

Ogawa, H .

FEBS LETTERS, 2004, 572 (1-3) :57-64