Isolation of a Metabolite from the pks Island Provides Insights into Colibactin Biosynthesis and Activity

被引：56

作者：

Brotherton, Carolyn A. ^{[1
]}

Wilson, Matthew ^{[1
]}

Byrd, Gary ^{[2
]}

Balskus, Emily P. ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[2] Harvard Univ, Small Mol Mass Spectrometry Facil, Fac Arts & Sci, Div Sci, Cambridge, MA 02138 USA

来源：

ORGANIC LETTERS | 2015年 / 17卷 / 06期

关键词：

IN-VIVO; DNA; DERIVATIVES; ACTIVATION; CHEMISTRY; MECHANISM; CC-1065;

D O I：

10.1021/acs.orglett.5b00432

中图分类号：

O62 [有机化学];

学科分类号：

070303 [有机化学];

摘要：

Colibactin is a structurally uncharacterized, genotoxic natural product produced by commensal and pathogenic strains of E. coli that harbor the pks island. A new metabolite has been isolated from a pks(+) E. coli mutant missing an essential biosynthetic enzyme. The unusual azaspiro[2.4] bicyclic ring system of this molecule provides new insights into colibactin biosynthesis and suggests a mechanism through which colibactin and other pks-derived metabolites may exert genotoxicity.

引用

页码：1545 / 1548

页数：4

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