Inhibition tests for detection and presumptive identification of tetracyclines, beta-lactam antibiotics and quinolones in poultry meat

被引:47
作者
Okerman, L
Croubels, S
De Baere, S
Van Hoof, J
De Backer, P
De Brabander, H
机构
[1] State Univ Ghent, Fac Med Vet, Dept Vet Food Inspect, B-9820 Merelbeke, Belgium
[2] State Univ Ghent, Fac Med Vet, Dept Phamracol Pharm & Toxicol, B-9820 Merelbeke, Belgium
来源
FOOD ADDITIVES AND CONTAMINANTS | 2001年 / 18卷 / 05期
关键词
antibitiotic residue; poultry; inhibition test;
D O I
10.1080/02652030010024320
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A combination of three plates, seeded with strains of Micrococcus luteus, Bacillus cereus or Escherichia coli, can be used for detection of residues of betalactam antibiot ics, tetracyclines and fluoroquinolones. The sensitivity of each plate is optimal for only one of these groups, resulting in detection limits (LOD) lower than the corresponding maxim um residue limits (MRL) and in distinct inhibition patterns typical for each antibiotic family. Beta-lactam antibiot ics such as penicillin G, ampicillin and amoxicillin give only inhibition zones on the plate with M. luteus. Tetracyclines are detected up to the MRL level with B. cereus, and fluoroquinolones with E. coli. The LODs of the antibiotics tested were as follows: penicillin G (PENG) 0.9 ng, ampicillin (AMPI) 0.6 ng and amoxicillin (AMOX) 1.0 ng on the plate with M. luteus; tetracycline (TET) 4 ng, oxytetracycline (OXY) 3 ng, doxycycline (DOX) 0.6 ng, and chlortetracycline (CHL) 0.3 ng on the plate with B. cereus; enrofloxacin (ENRX) 1.5 ng, ciprofloxacin (CIPX) 0.5 ng and flumequine (FLUM) 1.5 ng on the plate with E. coli. The combination of plates enables the laboratory to select appropriate chromatographic techniques for identification and quantification of the residues. On the other hand, the three groups can also be detected on one plate seeded with Bacillus subtilis, although the limits of detection are higher: PENG 0.4 ng, AMPI and AMOX 3 ng; TET 5 ng, OXY 8 ng, DOX 1 ng, CHL 0.5 ng, ENRX 4 ng, CIPX 10 ng and FLUM 4 ng. The test was applied to 228 broiler fillets and to 27 turkey thighs, originating from different poultry slaughterhouses. Nineteen broiler fillets contained inhibiting substances. The positive results of the inhibition tests were confirmed with a chromatographic technique. Doxycycline residues were found in 16 samples and amoxicillin in two.
引用
收藏
页码:385 / 393
页数:9
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