A Comparative Lipidomics Platform for Chemotaxonomic Analysis of Mycobacterium tuberculosis

被引：153

作者：

Layre, Emilie ^{[1
]}

Sweet, Lindsay ^{[1
]}

Hong, Sunhee ^{[1
]}

Madigan, Cressida A. ^{[1
]}

Desjardins, Danielle ^{[1
]}

Young, David C. ^{[1
]}

Cheng, Tan-Yun ^{[1
]}

Armand, John W. ^{[1
]}

Kim, Keunpyo ^{[2
]}

Shamputa, Isdore C. ^{[3
]}

McConnell, Matthew J. ^{[1
]}

Debono, C. Anthony ^{[1
]}

Behar, Samuel M. ^{[1
]}

Minnaard, Adriaan J. ^{[4
]}

Murray, Megan ^{[5
]}

Barry, Clifton E., III ^{[3
]}

Matsunaga, Isamu ^{[6
]}

Moody, D. Branch ^{[1
]}

机构：

[1] Harvard Univ, Brigham & Womens Hosp, Sch Med, Div Rheumatol Immunol & Allergy, Boston, MA 02115 USA

[2] Medimmune Inc, Biostat, Gaithersburg, MD 20878 USA

[3] NIAID, TB Res Sect, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA

[4] Univ Groningen, Stratingh Inst Chem, NL-9747 AG Groningen, Netherlands

[5] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA

[6] Kyoto Univ, Dept Viral Oncol, Lab Cell Regulat, Inst Virus Res, Kyoto 6068507, Japan

来源：

CHEMISTRY & BIOLOGY | 2011年 / 18卷 / 12期

基金：

美国国家卫生研究院;

关键词：

TANDEM MASS-SPECTROMETRY; OUTER-MEMBRANE; STRAINS; LIPIDS; INFECTION; VIRULENCE; IDENTIFICATION; REVEALS; COMPLEX; PHENOLGLYCOLIPIDS;

D O I：

10.1016/j.chembiol.2011.10.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lipidic envelope of Mycobacterium tuberculosis promotes virulence in many ways, so we developed a lipidomics platform for a broad survey of cell walls. Here we report two new databases (MycoMass, MycoMap), 30 lipid fine maps, and mass spectrometry datasets that comprise a static lipidome. Further, by rapidly regenerating lipidomic datasets during biological processes, comparative lipidomics provides statistically valid, organism-wide comparisons that broadly assess lipid changes during infection or among clinical strains of mycobacteria. Using stringent data filters, we tracked more than 5,000 molecular features in parallel with few or no false-positive molecular discoveries. The low error rates allowed chemotaxonomic analyses of mycobacteria, which describe the extent of chemical change in each strain and identified particular strain-specific molecules for use as biomarkers.

引用

页码：1537 / 1549

页数：13

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