A new approach for creating double-stranded DNA biosensors

被引：17

作者：

Skuridin, SG

Yevdokimov, YM

Efimov, VS

Hall, JM

Turner, APF

机构：

[1] RUSSIAN ACAD SCI,VA ENGELHARDT MOLEC BIOL INST,MOSCOW 117984,RUSSIA

[2] RUSSIAN STATE MED UNIV,MOSCOW 117869,RUSSIA

[3] CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND

来源：

BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 09期

关键词：

biosensors; DNA; DNA liquid-crystalline dispersion; polycations; DNA-polycation] complexes; intermolecular crosslinks; protamines; trypsin; CD spectra;

D O I：

10.1016/0956-5663(96)89439-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The principle of 'sandwich'-type biosensors based on liquid-crystalline dispersions formed from [DNA-polycation] complexes is outlined. These biosensors will find application in the determination of a range of compounds and physical factors that affect the ability of a given polycationic molecule to maintain intermolecular crosslinks between neighbouring DNA molecules. In the case of liquid-crystalline dispersions formed from [DNA-protamine] complexes the lowest concentration of hydrolytic enzyme (trypsin) detectable was approximate to-10(-14) M. (C) 1996 Elsevier Science Limited.

引用

页码：903 / 911

页数：9

共 21 条

[1] COMPACT FORM OF METHYLATED DNA IN SOLUTIONS CONTAINING POLY (ETHYLENE-GLYCOL) [J].