Identification of moulds in the diagnostic laboratory -: an algorithm implementing molecular and phenotypic methods

被引：33

作者：

Ciardo, Diana E. ^{[1
]}

Schaer, Gertrud ^{[1
]}

Altwegg, Martin ^{[1
]}

Boerrger, Erik C. ^{[1
]}

Bosshard, Philipp P. ^{[1
]}

机构：

[1] Univ Zurich, Inst Med Mikrobiol, CH-8006 Zurich, Switzerland

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2007年 / 59卷 / 01期

关键词：

moulds; ITS; identification; Sequencing; PCR; fungi; algorithm; diagnostics;

D O I：

10.1016/j.diagmicrobio.2007.04.020

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Sequence analysis provides a valuable alternative to phenotypic identification of moulds, especially for isolates lacking characteristic morphology. In this comparative prospective study, isolates that could not be identified by standard phenotypic criteria within 5 days (n = 244) were subjected to sequence analysis and further in-depth phenotypic investigations. Comparison of sequence-based with extended phenotypic identification revealed that sequence analysis was more precise in 52.0% of the isolates; in 38.6% of the isolates, both methods gave concordant results. The construction of a database consisting of high-quality sequences allowed improvement of sequence-based identification. Based on these results, we propose a diagnostic algorithm for the effective use of both phenotypic and genetic procedures for identification of moulds in the diagnostic laboratory. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：49 / 60

页数：12

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