Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS

被引：52

作者：

Benfante, Roberta ^{[1
,2
,3
]}

Antonini, Ruth Adele ^{[1
,2
,3
]}

De Pizzol, Maria ^{[4
,5
]}

Gotti, Cecilia ^{[1
,2
,3
]}

Clementi, Francesco ^{[1
,2
,3
]}

Locati, Massimo ^{[4
,5
]}

Fornasari, Diego ^{[1
,2
,3
]}

机构：

[1] Univ Milan, Sch Med, Dept Pharmacol, I-20129 Milan, Italy

[2] CNR, Inst Neurosci, I-20133 Milan, Italy

[3] Univ Milan, CEND Ctr Excellence Study Neurodegenerat Dis, I-20129 Milan, Italy

[4] Univ Milan, Dept Translat Med, I-20129 Milan, Italy

[5] IRCCS Ist Clin Humanitas, Milan, Italy

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2011年 / 230卷 / 1-2期

关键词：

Nicotinic acetylcholine receptor; CHRFAM7A; CHRNA7; LPS; Monocytes; NF-kappa B; NICOTINIC ACETYLCHOLINE-RECEPTOR; TRANSCRIPTION FACTOR EGR-1; KAPPA-B KINASE; VAGUS NERVE; NEURODEGENERATIVE DISEASE; RHEUMATOID-ARTHRITIS; COPY NUMBER; WHOLE-BLOOD; GENE CHRNA7; INFLAMMATION;

D O I：

10.1016/j.jneuroim.2010.09.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We investigated the expression of the partially duplicated alpha 7 nAChR subunit gene in three monocytic cell lines: THP-1, U937 and Mono-Mac-6. Qualitative PCR revealed the presence of the classic alpha 7 gene in these lineages, but real-time PCR showed the exclusive expression of alpha 7dup. Both mRNA and protein levels were reduced in THP-1 upon LPS challenge, and it was found that transcriptional down-regulation was mediated by a direct mechanism dependent on NF-kappa B as its specific inhibitor parthenolide prevented the reduction in the alpha 7dup transcript Such precise regulation suggests that alpha 7dup may specifically participate in the inflammatory response of the innate immune system. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：74 / 84

页数：11

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[1] Whole blood cytokine attenuation by cholinergic agonists ex vivo and relationship to vagus nerve activity in rheumatoid arthritis [J].