Comparison of cytotoxicity of aminoglycoside antibiotics using a panel cellular biotest system

被引：22

作者：

Chernikov, VG ^{[1
]}

Terekhov, SM ^{[1
]}

Krokhina, TB ^{[1
]}

Shishkin, SS ^{[1
]}

Smirnova, TD ^{[1
]}

Kalashnikova, EA ^{[1
]}

Adnoral, NV ^{[1
]}

Rebrov, LB ^{[1
]}

Denisov-Nikol'skii, YI ^{[1
]}

Bykov, VA ^{[1
]}

机构：

[1] Russian Acad Med Sci, Med Genet Res Ctr, Res Ctr Biomed Technol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2003年 / 135卷 / 01期

关键词：

human cell cultures; biotest system; aminoglycoside antibiotics; cytotoxicity;

D O I：

10.1023/A:1023474719042

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

The cytotoxicity of four aminoglycoside antibiotics was studied by estimation of the dose-effect relationship using a panel cellular biotest system including cell cultures for test objects. The cultures represented 4 differentiation types: normal human fibroblasts and myoblasts, human or Syrian hamster hepatoma cells, and mouse/mouse hybridoma cells. It was found that three widely used antibiotics gentamicin, kanamycin, and neomycin exhibit similar, but not identical cytotoxicity parameters and differ distinctly from geneticin. Hence, the proposed panel biotest system helps to quantitatively evaluate and differentiate the effects of bioactive substances with similar chemical structure.

引用

页码：103 / 105

页数：3

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