Locating the petunia Rf gene on a 650-kb DNA fragment

被引：19

作者：

Bentolila, S

Zethof, J

Gerats, T

Hanson, MR

机构：

[1] Cornell Univ, Genet & Dev Sect, Ithaca, NY 14853 USA

[2] State Univ Ghent, Genet Lab, B-9000 Ghent, Belgium

来源：

THEORETICAL AND APPLIED GENETICS | 1998年 / 96卷 / 6-7期

关键词：

petunia; Rf; molecular marker; mapping; synteny;

D O I：

10.1007/s001220050829

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A bulked segregant analysis was conducted in order to find RAPD and AFLP markers linked to the restorer of fertility (Rf) gene in petunia. One RAPD marker, OP704, and one AFLP marker, ECCA/ MACT, were found to be closely linked to Rf(<1 cM) in our mapping population produced from an intraspecific Petunia hybrida cross. These two single-copy markers bracketing Rf were then mapped as RFLPs on the tomato map. Despite some rearrangement: between the petunia and the tomato genomes, this synteny survey revealed two tomato markers, TG250 and CT24, closely linked to Rf: Physical mapping indicates that CT24, OP704 and ECCA/MACT lie on the same 650-kb Milli fragment. A physical to genetic distance ratio of 400 kb/cM around the Rf gene should make it feasible to identify markers physically very close to Rf.

引用

页码：980 / 988

页数：9

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