Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1) modulates the acouisition of cisplatin resistance in bladder cancer cell lines

被引：61

作者：

Tsunoda, T ^{[1
]}

Koga, H ^{[1
]}

Yokomizo, A ^{[1
]}

Tatsugami, K ^{[1
]}

Eto, M ^{[1
]}

Inokuchi, J ^{[1
]}

Hirata, A ^{[1
]}

Masuda, K ^{[1
]}

Okumura, K ^{[1
]}

Naito, S ^{[1
]}

机构：

[1] Kyushu Univ, Dept Urol, Grad Sch Med Sci, Higashi Ku, Fukuoka 8128582, Japan

来源：

ONCOGENE | 2005年 / 24卷 / 08期

关键词：

cisplatin resistance; IP(3)R1; apoptosis; bladder; cancer;

D O I：

10.1038/sj.onc.1208313

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (11) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor typel (IP(3)R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression Of IP(3)R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression Of IP(3)R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP(3)R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells. Published online 20 December 2004.

引用

页码：1396 / 1402

页数：7

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