Production of prednisolone by Bacillus pumilus E601 cells incorporated in radiation induced poly(vinyl alcohol g-2 hydroxyethylmethacrylate) cryogels

被引:5
作者
El-Hadi, AA [1 ]
机构
[1] Natl Res Ctr, Dept Chem Nat & Microbial Prod, Cairo, Egypt
关键词
prednisolone; Bacillus pumilus; radiation induced cryogels;
D O I
10.1016/S0032-9592(02)00101-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Immobilization of Bacillus pumilus E601 in poly(vinyl alcohol) (PVA) cryogels at a concentration of 10% grafted with hydroxyethylmethacrylate (HEMA) at a concentration of 0.4% using a radiation polymerization technique lead to an increase in the prednisolone yield (46%) compared with the prednisolone yield (38%) produced by immobilized B. pumilus E601 carrier on the surface of the polymer at the same concentrations. The Delta(-1)-dehydrogenase of B. pumilus E601 was affected by the molecular weight, the irradiation dose and the diameter of the polymer. The storage of immobilized B. pumilus E601 in poly(PVA)/HEMA at -4 degreesC for 30 days shows a higher yield of prednisolone (80%) as compared with prednisolone yield (75%) at 25 degreesC at the time of storage. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1653 / 1657
页数:5
相关论文
共 13 条
[1]   SYNTHETIC HYDROGELS .6. HYDROGEL COMPOSITES AS WOUND DRESSINGS AND IMPLANT MATERIALS [J].
CORKHILL, PH ;
HAMILTON, CJ ;
TIGHE, BJ .
BIOMATERIALS, 1989, 10 (01) :3-10
[2]  
FOKINA VV, 1995, APPL BIOCHEM MICRO+, V31, P184
[3]  
HIGA OZ, 1986, RADIAT PHYS CHEM, V27, P311
[4]   IMMOBILIZATION OF MICROBIAL-CELLS ON CELLULOSE POLYMER SURFACES BY RADIATION POLYMERIZATION [J].
KUMAKURA, M ;
KAETSU, I .
JOURNAL OF APPLIED POLYMER SCIENCE, 1983, 28 (12) :3759-3765
[5]   Poly(vinyl alcohol) cryogels employed as matrices for cell immobilization .2. Entrapped cells resemble porous fillers in their effects on the properties of PVA-cryogel carrier [J].
Lozinsky, VI ;
Zubov, AL ;
Titova, EF .
ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (03) :182-190
[6]  
NORTON S, 1994, ENZYME MICROB TECHNO, V16, P363
[7]   IMMOBILIZATION OF ARTHROBACTER-SIMPLEX IN A THERMALLY REVERSIBLE HYDROGEL - EFFECT OF TEMPERATURE CYCLING ON STEROID CONVERSION [J].
PARK, TG ;
HOFFMAN, AS .
BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (02) :152-159
[8]  
Peppas N., 1987, Hydrogels in Medicine and Pharmacy
[9]  
Pizarro C, 1997, BIOTECHNOL BIOENG, V53, P497
[10]   The characterization of dressing component materials and radiation formation of PVA-PVP hydrogel [J].
Razzak, MT ;
Zainuddin ;
Erizal ;
Dewi, SP ;
Lely, H ;
Taty, E ;
Sukirno .
RADIATION PHYSICS AND CHEMISTRY, 1999, 55 (02) :153-165