New approaches to structure determination by NMR spectroscopy

被引：27

作者：

Dötsch, V ^{[1
]}

Wagner, G ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1998年 / 8卷 / 05期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/S0959-440X(98)80154-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New strategies have recently been developed for studying biological macromolecules of large size (beyond 100 kDa) in order to both improve the quality of the structures and make structure determination more efficient. This has been achieved by utilizing cross-correlation effects and novel labeling strategies, and developing novel NMR spectroscopy experiments.

引用

页码：619 / 623

页数：5

共 37 条

[1] High-resolution heteronuclear NMR of human ubiquitin in an aqueous liquid crystalline medium [J].

Bax, A ;

Tjandra, N .

JOURNAL OF BIOMOLECULAR NMR, 1997, 10 (03) :289-292

[2] Direct structure refinement against residual dipolar couplings in the presence of rhombicity of unknown magnitude [J].

Clore, GM ;

Gronenborn, AM ;

Tjandra, N .

JOURNAL OF MAGNETIC RESONANCE, 1998, 131 (01) :159-162

[3] Dimerization of the UmuD' protein in solution and its implications for regulation of SOS mutagenesis [J].

Ferentz, AE ;

Opperman, T ;

Walker, GC ;

Wagner, G .

NATURE STRUCTURAL BIOLOGY, 1997, 4 (12) :979-983

[4] DIPOLAR MAGNETIC-FIELD EFFECTS IN NMR-SPECTRA OF LIQUIDS [J].

GAYATHRI, C ;

BOTHNERBY, AA ;

VANZIJL, PCM ;

MACLEAN, C .

CHEMICAL PHYSICS LETTERS, 1982, 87 (02) :192-196

[5] Broadband adiabatic refocusing without phase distortion [J].

Hwang, TL ;

van Zijl, PCM ;

Garwood, M .

JOURNAL OF MAGNETIC RESONANCE, 1997, 124 (01) :250-254

[6]

JASANOFF A, IN PRESS J BIOMOL NM

[7] Solution NMR spectroscopy beyond 25 kDa [J].

Kay, LE ;

Gardner, KH .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1997, 7 (05) :722-731

[8] Compensation for spin-spin coupling effects during adiabatic pulses [J].

Kupce, E ;

Freeman, R .

JOURNAL OF MAGNETIC RESONANCE, 1997, 127 (01) :36-48

[9] Multisite band-selective decoupling in proteins [J].

Kupce, E ;

Wagner, G .

JOURNAL OF MAGNETIC RESONANCE SERIES B, 1996, 110 (03) :309-312

[10] Dynamical mapping of E-coli thioredoxin via C-13 NMR relaxation analysis [J].

LeMaster, DM ;

Kushlan, DM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (39) :9255-9264

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