Type-specific detection of echovirus 30 isolates using degenerate reverse transcriptase PCR primers

被引:23
作者
Kilpatrick, DR [1 ]
Quay, J [1 ]
Pallansch, MA [1 ]
Oberste, MS [1 ]
机构
[1] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Viral & Rickettsial Dis, Resp & Enter Viruses Branch, Atlanta, GA 30333 USA
关键词
D O I
10.1128/JCM.39.4.1299-1302.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Following an approach used to specifically identify polioviruses and enterovirus 71, we have developed reverse transcriptase (RT) PCR primers containing mixed-base residues or deoxyinosine at positions of codon degeneracy. These printers permit specific RT-PCR amplification of echovirus 30 (E30) sequences by targeting sites that encode conserved amino acid motifs within the major capsid protein, VPI. All 221 E30 strains tested, isolated in 16 countries over a 44-year period, yielded the predicted 158-bp PCR product, No specific products were obtained by PCR assays containing templates from any of the other 63 EV serotypes, Inosine-containing degenerate primers may be widely applicable to the identification of echovirus serotypes by PCR.
引用
收藏
页码:1299 / 1302
页数:4
相关论文
共 37 条
[1]  
[Anonymous], 1997, Morbidity and Mortality Weekly Report, V46, P748
[2]  
[Anonymous], 1957, AM J PUBLIC HEALTH N, V47, P1556, DOI 10.2105/AJPH.47.12.1556
[3]  
ARCHARYA R, 1989, NATURE, V338, P709
[4]   Natural isolates of ECHO virus type 25 with extensive variations in IRES sequences and different translational efficiencies [J].
Bailly, JL ;
Borman, AM ;
PeigueLafeuille, H ;
Kean, KM .
VIROLOGY, 1996, 215 (01) :83-96
[5]   Serotype-specific identification of enterovirus 71 by PCR [J].
Brown, BA ;
Kilpatrick, DR ;
Oberste, MS ;
Pallansch, MA .
JOURNAL OF CLINICAL VIROLOGY, 2000, 16 (02) :107-112
[6]  
DUNCAN IB, 1968, GESAMTE VIRUSFORSCH, V25, P93
[7]  
GRANDIEN M, 1989, DIAGNOSTIC PROCEDURE, P513
[8]   3-DIMENSIONAL STRUCTURE OF POLIOVIRUS AT 2.9 A RESOLUTION [J].
HOGLE, JM ;
CHOW, M ;
FILMAN, DJ .
SCIENCE, 1985, 229 (4720) :1358-1365
[9]   Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residues at positions of codon degeneracy [J].
Kilpatrick, DR ;
Nottay, B ;
Yang, CF ;
Yang, SJ ;
Mulders, MN ;
Holloway, BP ;
Pallansch, MA ;
Kew, OM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (12) :2990-2996
[10]   Serotype-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residues at positions of codon degeneracy [J].
Kilpatrick, DR ;
Nottay, B ;
Yang, CF ;
Yang, SJ ;
Da Silva, E ;
Peñaranda, S ;
Pallansch, M ;
Kew, O .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (02) :352-357