Salsolinol is present in the bovine posterior pituitary gland and stimulates the release of prolactin both in vivo and in vitro in ruminants

被引：27

作者：

Hashizume, T. ^{[1
]}

Shida, R. ^{[1
]}

Suzuki, S. ^{[1
]}

Nonaka, S. ^{[1
]}

Yonezawa, C. ^{[1
]}

Yamashita, T. ^{[1
]}

Kasuya, E. ^{[2
]}

Sutoh, M. ^{[3
]}

Olah, M. ^{[4
,5
]}

Szekacs, D. ^{[4
,5
]}

Nagy, G. M. ^{[4
,5
]}

机构：

[1] Iwate Univ, Fac Agr, Morioka, Iwate 0208550, Japan

[2] Natl Inst Agrobiol Sci, Lab Anim Neurophysiol, Tsukuba, Ibaraki 3508550, Japan

[3] Natl Inst Livestock & Grassland Sci, Lab Growth Regulat, Tsukuba, Ibaraki 3050901, Japan

[4] Hungarian Acad Sci, Dept Human Morphol, Neuroendrocrine Res Lab, Budapest, Hungary

[5] Semmelweis Univ, H-1085 Budapest, Hungary

来源：

DOMESTIC ANIMAL ENDOCRINOLOGY | 2008年 / 34卷 / 02期

关键词：

salsolinol; prolactin; ruminants;

D O I：

10.1016/j.domaniend.2006.12.003

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aims of the present study were to determine whether salsolinol (SAL), a dopamine-related compound, is present in the bovine posterior pituitary (PP) gland, and to clarify the effect of SAL on the secretion of prolactin (PRL) in ruminants. SAL was detected in extract of bovine PP gland using high-pressure liquid chromatography with electrochemical detection (HPLC-EC). A single intravenous (i.v.) injection of SAL (5 and 10 mg/kg body weight) significantly and dose-dependently stimulated the release of PRL in goats (P < 0.05). Plasma PRL levels reached a peak 10 min after the injection, then gradually returned to basal values in 60-80 min. The PRL-releasing pattern was similar to that in response to sulpiride (a dopamine receptor antagonist). The intracerebroventricular (i.c.v.) injection of I mg of SAL had no significant effect on the release of PRL in calves, however, 5 mg significantly stimulated the release (P < 0.05) with peak values reached 30-40 min after the injection. Moreover, SAL significantly stimulated the release of PRL from cultured bovine anterior pituitary cells at doses of 10(-6) and 10(-5) M, compared to control cells (P < 0.05). Taken together, our data clearly show that SAL is present in extract of the PP gland of ruminants, and has PRL-releasing activity both in vivo and in vitro. Therefore, this endogenous compound is a strong candidate for the factor having PRL-releasing activity that has been previously detected in extract of the bovine PP gland. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：146 / 152

页数：7

共 14 条

[1] Stress- as well as suckling-induced prolactin release is blocked by a structural analogue of the putative hypophysiotrophic prolactin-releasing factor, salsolinol [J].