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Karyotyping mouse chromosomes by multiplex-FISH (M-FISH)

被引：26
作者：
Jentsch, I
Adler, ID
Carter, NP
Speicher, MR
机构：
[1] Univ Munich, Inst Anthropol & Human Genet, D-80336 Munich, Germany
[2] GSF, Natl Res Ctr Environm & Hlth, D-85764 Neuherberg, Germany
[3] Sanger Ctr, Cambridge CB10 1SA, England
来源：
CHROMOSOME RESEARCH | 2001年 / 9卷 / 03期
关键词：
fluorescence in situ hybridization (FISH); karyotyping; mouse chromosomes; multiplex-FISH (M-FISH); translocations;
D O I：
10.1023/A:1016696303479
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Karyotyping of mouse chromosomes is a skillful art, which is laborious work even for experienced cytogeneticists. With the growing number of mouse models for human diseases, there is an increasing demand for automated mouse karyotyping systems. Here, such a karyotyping system for mouse chromosomes based on the multiplex-fluorescence in-situ hybridization (M-FISH) technology is shown. The system was tested on a number of individual mice with numerical and structural aberrations and its reproducibility and robustness verified. Mouse M-FISH should be a valuable tool for the analysis of chromosomal rearrangements in mice.
引用
收藏
页码：211 / 214
页数：4
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