Genotoxicity of selected pesticides in the mouse bone-marrow micronucleus test and in the sister-chromatid exchange test with human lymphocytes in vitro

被引：46

作者：

Kevekordes, S ^{[1
]}

Gebel, T ^{[1
]}

Pav, K ^{[1
]}

Edenharder, R ^{[1
]}

Dunkelberg, H ^{[1
]}

机构：

[1] UNIV MAINZ, DEPT HYG, INST MED MICROBIOL & HYG, D-55131 MAINZ, GERMANY

来源：

TOXICOLOGY LETTERS | 1996年 / 89卷 / 01期

关键词：

micronucleus test (MNT) in vivo; bone-marrow; sister-chromatid exchange (SCE) in vitro; human lymphocytes; genotoxicity; pesticides;

D O I：

10.1016/S0378-4274(96)03779-4

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Selected pesticides (aldicarb, 1,3-dichloropropene, methidathion, parathion, triadimefon, vinclozolin) were tested for their clastogenic and aneugenic activities in the mouse bone-marrow micronucleus (MN) test in vivo and for their sister-chromatid exchange-inducing activities in human lymphocytes in vitro in the presence and absence of an exogenous metabolizing system from rat-liver S9. 1,3-Dichloropropene significantly increased the frequencies of micronucleated polychromatic erythrocytes (PCE) in bone-marrow cells of female mice from 3.3 MN/1000 PCE to. 15.3 MN/1000 PCE (187 mg per kg body weight). 1,3-Dichloropropene (100 mu M) induced 16.0 SCE/metaphase after 24 h of incubation as compared with the basal rate of 11.2 SCE/metaphase (-S9) and of 15.4 SCE/metaphase as compared with 10.5 SCE/metaphase of the control (+S9). These values were statistically significantly different from each other. The other pesticides tested did neither increase the rate of micronuclei significantly in polychromatic erythrocytes in male nor in female animals. Aldicarb and methidathion induced a significant increase in SCEs in human lymphocytes in vitro only without the metabolic activating system: aldicarb, 5 mu M, 24 h incubation: 15.5 SCE/metaphase; control: 12.6 SCE/metaphase; methidathion, 100 mu M, 24 h incubation: 15.8 SCE/metaphase, control: 11.1 SCE/metaphase. Parathion, triadimefon and vinclozolin did not have any SCE-inducing effects.

引用

页码：35 / 42

页数：8

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