Expression of class I O-methyltransferase in healthy and TMV-infected tobacco

Tobacco possesses two distinct classes of O-methltransferases (OMTs; S-adenosyl-L-methionine :o-diphenol O-methyltransferases; EC 2.1.1.6). Here we report on the cloning and the expression pattern of the class I OMT that is specifically involved in lignin biosynthesis. Near-full-length cDNAs have been isolated from tobacco libraries constructed from leaf and stem poly(A)(+) RNA. Sequence analysis demonstrated that the OMT I clones derived from two different mRNA species, The two types of OMT I mRNA were reverse transcribed from total RNA and the cDNAs were amplified by polymerase chain reaction and characterized by restriction analysis, The same proportion of the two transcripts was measured in stem tissue of healthy plants and in leaves reacting hypersensitively to tobacco mosaic virus, indicating a coordinate expression of the two OMT I genes. Consistently, genomic hybridization indicated the presence of two OMT I genes in the amphidiploid genome of tobacco, The pattern, of expression of OMT I genes was studied by in situ mRNA hybridization, In stem, petiole, and root tissues, OMT I genes were found to be specifically expressed in vascular cells and epidermis, In healthy leaves OMT I mRNA was only detected in vascular strands, whereas, in leaves bearing tobacco mosaic virus-induced necrotic lesions, a particularly strong accumulation of the labeling was also localized in the upper and lower epidermis.