Discrimination of liver cirrhosis from chronic hepatitis by measuring the ratio of Aleuria aurantia lectin-reactive serum cholinesterase to immunoreactive protein

被引:15
作者
Hada, T [1 ]
Kondo, M
Yasukawa, K
Amuro, Y
Higashino, K
机构
[1] Hyogo Coll Med, Dept Internal Med 3, Nishinomiya, Hyogo 663, Japan
[2] Tosoh Corp, Tokyo Res Ctr, Ayase, Kanagawa 252, Japan
关键词
lectin; sugar chain; chronic liver disease; ELISA;
D O I
10.1016/S0009-8981(98)00202-2
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
We have previously reported that Aleuria aurantia lectin (AAL)-reactive serum cholinesterase (ChE) activity increases in liver cirrhosis (LC) and hepatocellular carcinoma (HCC) compared with chronic hepatitis (CH) and normal controls (NC), and measurement of AAL-reactive ChE activity is useful in discriminating LC from CH. In the present study, Ive have demonstrated that the measurement of the ratio of AAL-reactive ChE to immuno-reactive ChE protein (AAL/ChE) is superior to the measurement of only AAL-reactive ChE for differentiating LC from CH. At a cut-off value of 4.0 arbitrary units of AAL/ChE, the diagnostic accuracy was 87.7%. This diagnostic accuracy is similar to that of serum hyaluronan, 88.8%. We also examined whether the AAL/ChE measurement is useful for differentiating Child's stage A LC from chronic active hepatitis (CAH) 2B. When mean + 2SD of AAL/ChE in patients with CAH2B was used as a cut-off value for the specific diagnosis of LC, the diagnostic accuracy was 70.2%. These results demonstrate that measurement of AAL/ChE is useful for discriminating LC from CH. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:37 / 46
页数:10
相关论文
共 14 条
[1]  
DEGROOTE J, 1968, LANCET, V2, P626
[2]  
Guechot J, 1996, CLIN CHEM, V42, P558
[3]   INCREASED PLASMA ALPHA(1-]3)-L-FUCOSYL-TRANSFERASE ACTIVITIES IN PATIENTS WITH HEPATOCELLULAR-CARCINOMA [J].
HADA, T ;
FUKUI, K ;
OHNO, M ;
AKAMATSU, S ;
YAZAWA, S ;
ENOMOTO, K ;
YAMAGUCHI, K ;
MATSUDA, Y ;
AMURO, Y ;
YAMANAKA, N ;
OKAMOTO, E ;
HIGASHINO, K .
GLYCOCONJUGATE JOURNAL, 1995, 12 (05) :627-631
[4]   Human T-cell leukemia virus-1 encoded tax protein transactivates alpha 1->3 fucosyltransferase Fuc-T VII, which synthesizes sialyl Lewis X, a serectin ligand expressed on adult T-cell leukemia cells [J].
Hiraiwa, N ;
Hiraiwa, M ;
Kannagi, R .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 231 (01) :183-186
[5]  
HUTCHINSON WL, 1991, HEPATOLOGY, V13, P683, DOI 10.1016/0270-9139(91)92564-O
[6]   Transcriptional regulation of the N-acetylglucosaminyltransferase V gene in human bile duct carcinoma cells (HuCC-T1) is mediated by Ets-1 [J].
Kang, R ;
Saito, H ;
Ihara, Y ;
Miyoshi, E ;
Koyama, N ;
Sheng, Y ;
Taniguchi, N .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (43) :26706-26712
[7]  
KOBAYASHI K, 1991, GANN MONOGR CANC RES, V38, P53
[8]   Enzyme-linked immunosorbent assay (ELISA) for Aleuria aurantia lectin-reactive serum cholinesterase to differentiate liver cirrhosis and chronic hepatitis [J].
Kondo, M ;
Hada, T ;
Fukui, K ;
Iwasaki, A ;
Higashino, K ;
Yasukawa, K .
CLINICA CHIMICA ACTA, 1995, 243 (01) :1-9
[9]   INDUCTION OF N-ACETYLGLUCOSAMINYLTRANSFERASE-V BY ELEVATED EXPRESSION OF ACTIVATED-HA-RAS ONCOGENES OR PROTO-HA-RAS ONCOGENES [J].
LU, Y ;
CHANEY, W .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 1993, 122 (01) :85-92
[10]   Clinical significance of serum hyaluronan in patients with chronic viral liver disease [J].
Murawaki, Y ;
Ikuta, Y ;
Koda, M ;
Nishimura, Y ;
Kawasaki, H .
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 1996, 11 (05) :459-465