Pivotal role of cyclic nucleoside phosphodiesterase 4 in Tat-mediated CD4+ T cell hyperactivation and HIV type 1 replication

被引：24

作者：

Secchiero, P

Zella, D

Curreli, S

Mirandola, P

Capitani, S

Gallo, RC

Zauli, G

机构：

[1] Univ Maryland, Inst Biotechnol, Inst Human Virol, Baltimore, MD 21201 USA

[2] Univ Ferrara, Dept Morphol & Embryol, Human Anat Sect, I-44100 Ferrara, Italy

[3] Univ G DAnnunzio, Inst Normal Human Morphol, I-66013 Chieti, Italy

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 26期

关键词：

D O I：

10.1073/pnas.011512398

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We show here that HIV type 1 (HIV-1)Tat protein, in combination with anti-CD3/CD28 mAbs, promotes IL-2 production and proliferation of primary CD4(+) T lymphocytes, obtained from HIV-1-seronegative donors. This effect was observed when Tat was immobilized on a solid support, but it was not observed with soluble Tat Such hyperactivation was accomplished by recruiting the rolipram-sensitive cyclic nucleoside phosphodiesterase 4 and resulted in increased susceptibility to HIV-1 infection. Accordingly, rolipram potently inhibited HIV-1 replication in cultures stimulated by anti-CD3/CD28 +/- Tat These results add to the concept that decreasing Tat activity is an important addition to anti-HIV-1 therapy, and they suggest a target for anti-HIV-1 chemotherapy, phosphodiesterase 4.

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页码：14620 / 14625

页数：6

相关论文

共 41 条

[1] HIV-1 Tat protein mimicry of chemokines [J].

Albini, A ;

Ferrini, S ;

Benelli, R ;

Sforzini, S ;

Giunciuglio, D ;

Aluigi, MG ;

Proudfoot, AEI ;

Alouani, S ;

Wells, TNC ;

Mariani, G ;

Rabin, RL ;

Farber, JM ;

Noonan, DM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (22) :13153-13158

[2] The angiogenesis induced by HIV-1 Tat protein is mediated by the Flk-1/KDR receptor on vascular endothelial cells [J].

Albini, A ;

Soldi, R ;

Giunciuglio, D ;

Giraudo, E ;

Benelli, R ;

Primo, L ;

Noonan, D ;

Salio, M ;

Camussi, G ;

Rockl, W ;

Bussolino, F .

NATURE MEDICINE, 1996, 2 (12) :1371-1375

[3]

ANASTASSIOU ED, 1992, J IMMUNOL, V148, P2845

[4] ROLIPRAM, A SPECIFIC TYPE-IV PHOSPHODIESTERASE INHIBITOR, IS A POTENT INHIBITOR OF HIV-1 REPLICATION [J].

ANGEL, JB ;

SAGET, BM ;

WALSH, SP ;

GRETEN, TF ;

DINARELLO, CA ;

SKOLNIK, PR ;

ENDRES, S .

AIDS, 1995, 9 (10) :1137-1144

[5] THE TAT PROTEIN OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1, A GROWTH-FACTOR FOR AIDS KAPOSI-SARCOMA AND CYTOKINE-ACTIVATED VASCULAR CELLS, INDUCES ADHESION OF THE SAME CELL-TYPES BY USING INTEGRIN RECEPTORS RECOGNIZING THE RGD AMINO-ACID-SEQUENCE [J].

BARILLARI, G ;

GENDELMAN, R ;

GALLO, RC ;

ENSOLI, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :7941-7945

[6] CYCLIC-NUCLEOTIDE PHOSPHODIESTERASES - FUNCTIONAL IMPLICATIONS OF MULTIPLE ISOFORMS [J].

BEAVO, JA .

PHYSIOLOGICAL REVIEWS, 1995, 75 (04) :725-748

[7] Extracellular HIV-1 Tat protein activates phosphatidylinositol 3- and Akt/PKB kinases in CD4(+) T lymphoblastoid Jurkat cells [J].

Borgatti, P ;

Zauli, G ;

Colamussi, ML ;

Gibellini, D ;

Previati, M ;

Cantley, LL ;

Capitani, S .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1997, 27 (11) :2805-2811

[8]

Boykins RA, 1999, J IMMUNOL, V163, P15

[9] Control of SHIV-89.6P-infection of cynomolgus monkeys by HIV-1 Tat protein vaccine [J].

Cafaro, A ;

Caputo, A ;

Fracasso, C ;

Maggiorella, MT ;

Goletti, D ;

Baroncelli, S ;

Pace, M ;

Sernicola, L ;

Koanga-Mogtomo, ML ;

Betti, M ;

Borsetti, A ;

Belli, R ;

Åkerblom, L ;

Corrias, F ;

Buttò, S ;

Heeney, J ;

Verani, P ;

Titti, F ;

Ensoli, B .

NATURE MEDICINE, 1999, 5 (06) :643-650

[10]

COFFEY RG, 1983, CANCER RES, V43, P150

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