Expression and anti-bacterial activity of human parotid secretory protein (PSP)

被引：48

作者：

Geetha, C ^{[1
]}

Venkatesh, SG ^{[1
]}

Dunn, BHF ^{[1
]}

Gorr, SU ^{[1
]}

机构：

[1] Univ Louisville, Sch Dent, Ctr Oral Hlth & System Dis, Louisville, KY 40292 USA

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2003年 / 31卷

关键词：

antibody; BPI (bactericidal/permeability-increasing protein); chromosome; 20; Pseudomonas aeruginosa; saliva;

D O I：

10.1042/bst0310815

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Parotid secretory protein (PSP) is an abundant protein in mouse and rat parotid glands. A related sequence (C20orf70) was identified on human chromosome 20. The goal of this study was to determine if PSP is expressed in the human parotid gland. The cDNA for human PSP was amplified from a human parotid cDNA sample. A peptide antibody, raised to the C-terminal peptide of PSP, identified the protein in human parotid tissue by immunofluorescence microscopy. Immunoaffinity chromatography suggested that PSP was expressed in human saliva. PSP is related to bactericidal/permeability-increasing protein (BPI). To test if PSP exhibits anti-bacterial activity, epitope-tagged PSP was expressed in rat GH4C1 cells. The secretion medium exhibited bacteristatic or bactericidal effects on Pseudomonas aeruginosa in a colony-forming assay when compared with secretion medium from GH4C1 cells that did not express PSP. These results suggest that PSP is expressed in the human parotid gland and saliva, where it functions as a BPI-like anti-bacterial protein.

引用

页码：815 / 818

页数：4

共 27 条

[1] PEPTIDE DERIVATIVES OF 3 DISTINCT LIPOPOLYSACCHARIDE-BINDING PROTEINS INHIBIT LIPOPOLYSACCHARIDE-INDUCED TUMOR-NECROSIS-FACTOR-ALPHA SECRETION IN-VITRO [J].

BATTAFARANO, RJ ;

DAHLBERG, PS ;

RATZ, CA ;

JOHNSTON, JW ;

GRAY, BH ;

HASEMAN, JR ;

MAYO, KH ;

DUNN, DL .

SURGERY, 1995, 118 (02) :318-324

[2] Detecting distant relatives of mammalian LPS-binding and lipid transport proteins [J].

Beamer, LJ ;

Fischer, D ;

Eisenberg, D .

PROTEIN SCIENCE, 1998, 7 (07) :1643-1646

[3] Characterisation of the human plunc gene, a gene product with an upper airways and nasopharyngeal restricted expression pattern [J].

Bingle, CD ;

Bingle, L .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2000, 1493 (03) :363-367

[4] PLUNC: A novel family of candidate host defence proteins expressed in the upper airways and nasopharynx [J].

Bingle, CD ;

Craven, CJ .

HUMAN MOLECULAR GENETICS, 2002, 11 (08) :937-943

[5] Progress in understanding autolmmune exocrinopathy using then on-obese diabetic mouse: An update [J].

Cha, S ;

Peck, AB ;

Humphreys-Beher, MG .

CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 2002, 13 (01) :5-16

[6] Sorting of an exocrine secretory protein to the regulated secretory pathway in endocrine cells [J].

Cowley, DJ ;

Chu, M ;

Gorr, SU .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 299 (01) :98-101

[7] Synthetic endotoxin-binding peptides block endotoxin-triggered TNF-α production by macrophages in vitro and in vivo and prevent endotoxin-mediated toxic shock [J].

Dankesreiter, S ;

Hoess, A ;

Schneider-Mergener, J ;

Wagner, H ;

Miethke, T .

JOURNAL OF IMMUNOLOGY, 2000, 164 (09) :4804-4811

[8] Molecular cloning and characterization of spurt, a human novel gene that is retinoic acid-inducible and encodes a secretory protein specific in upper respiratory tracts [J].

Di, YP ;

Harper, R ;

Zhao, YH ;

Pahlavan, N ;

Finkbeiner, W ;

Wu, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (02) :1165-1173

[9] Role of the bactericidal/permeability-increasing protein in host defence [J].

Elsbach, P ;

Weiss, J .

CURRENT OPINION IN IMMUNOLOGY, 1998, 10 (01) :45-49

[10]

Ghafouri B, 2002, PROTEOMICS, V2, P112, DOI 10.1002/1615-9861(200201)2:1<112::AID-PROT112>3.0.CO

← 1 2 3 →