Polymerase chain reaction for detection of legionellae DNA in urine samples from patients with community-acquired pneumonia

被引：8

作者：

Socan, M

Kese, D

Marinic-Fiser, N

机构：

[1] Univ Ljubljana, Med Ctr, Dept Infect Dis, Ljubljana 61000, Slovenia

[2] Univ Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana, Slovenia

[3] Inst Publ Hlth, Virol Lab, Ljubljana, Slovenia

来源：

FOLIA MICROBIOLOGICA | 2000年 / 45卷 / 05期

关键词：

D O I：

10.1007/BF02817623

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polymerase chain reaction (PCR) was used for detecting Legionella DNA in water, sputum, tracheal aspirate and bronchoalveolar lavage fluid. There is paucity of data on the use of PCR for detection of Legionella in serum and urine samples. In 82 patients admitted with community-acquired pneumonia, urinary PCR was used in addition to urinary antigen assay for Legionella pneumophila serogroup 1 and serological tests (indirect immunofluorescence and ELISA) in paired sera. PCR was positive in urine samples from 21 patients (26 %): in six of seven patients with acute legionellosis by CDC criteria, and 15 patients with negative urine antigen showing no fourfold rise in antibody titers in immunofluorescence test.

引用

页码：469 / 472

页数：4

共 24 条

[1] BACTERIOLOGICAL AND SEROLOGICAL DIAGNOSIS OF COMMUNITY-ACQUIRED ACUTE PNEUMONIA, SPECIALLY LEGIONNAIRES-DISEASE MULTICENTRIC PROSPECTIVE-STUDY OF 274 HOSPITALIZED-PATIENTS [J].

BORNSTEIN, N ;

FLEURETTE, J ;

BEBEAR, C ;

CHABANON, G .

ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY, 1987, 264 (1-2) :93-101

[2] False-positive Legionella titres in routine clinical serology testing detected by absorption with campylobacter: Implications for the serological diagnosis of Legionnaires' disease [J].