Enzymatic extraction of chondroitin sulfate from skate cartilage and concentration-desalting by ultrafiltration

被引：63

作者：

Lignot, B

Lahogue, V

Bourseau, P

机构：

[1] Univ Bretagne Sud, Ctr Rech, L2P, F-56325 Lorient, France

[2] ID Mer, F-56100 Lorient, France

来源：

JOURNAL OF BIOTECHNOLOGY | 2003年 / 103卷 / 03期

关键词：

glycosaminoglycan; enzymatic hydrolysis; desalting; ultrafiltration; marine by-products; upgrading;

D O I：

10.1016/S0168-1656(03)00139-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Skate cartilage is a fishery by-product, which contains chondroitin sulfate (CS), a glycosaminoglycan well known for its chondroprotective effect. Here described is a low-cost two-step process producing CS in non-denaturing conditions, consisting of an enzymatic extraction followed by tangential filtration to concentrate and purify CS. The performances of UF and MF membranes were compared in terms of flux and selectivity. The 0.1 mum-pore size membrane appeared to be the most efficient to separate CS from the other compounds. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：281 / 284

页数：4