Applicability of RNA standards for evaluating RT-qPCR assays and platforms

被引：29

作者：

Devonshire, Alison S. ^{[1
]}

Elaswarapu, Ramnath ^{[1
]}

Foy, Carole A. ^{[1
]}

机构：

[1] LGC Ltd, Teddington TW11 0LY, Middx, England

来源：

BMC GENOMICS | 2011年 / 12卷

关键词：

PCR;

D O I：

10.1186/1471-2164-12-118

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm (R) BioMark (TM)) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.

引用

页数：10

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