Hyperthermia enhances tumor necrosis factor α-induced apoptosis of a human gastric cancer cell line

被引：31

作者：

Kokura, S ^{[1
]}

Yoshida, N ^{[1
]}

Ueda, M ^{[1
]}

Imamoto, E ^{[1
]}

Ishikawa, T ^{[1
]}

Takagi, T ^{[1
]}

Naito, Y ^{[1
]}

Okanoue, T ^{[1
]}

Yoshikawa, T ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Dept Med 1, Kamigyo Ku, Mol Gastroenterol & Hepatol,Grad Sch Med Sci, Kyoto 6028566, Japan

来源：

CANCER LETTERS | 2003年 / 201卷 / 01期

关键词：

hyperthermia; tumor necrosis factor alpha; apoptosis; nuclear factor-kappa B;

D O I：

10.1016/S0304-3835(03)00463-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have investigated the effects of hyperthermia on the apoptosis induced by tumor necrosis factor alpha (TNF-alpha). Confluent monolayers of human gastric cancer cell line MKN45 were either treated or untreated with hyperthermia for I h. The cells were subsequently stimulated with TNF-alpha. A 24-h incubation with TNF-alpha did not affect cell viabilities; however, pretreatment with hyperthermia significantly enhanced the level of apoptosis induced by TNF-alpha. Pretreating MKN45 cells with hyperthermia (42.0 degreesC) significantly inhibited the TNF-alpha-induced increase in the binding activity of NF-kappaB to DNA. This study suggests that hyperthermia can inhibit the TNF-alpha-induced NF-kappaB activation and that hyperthermia renders human gastric cancer cells susceptible to the TNF-alpha-induced apoptosis, possibly via inhibition of the NF-kappaB pathway. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：89 / 96

页数：8

共 18 条

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