Wound healing of degree III furcation involvements following guided tissue regeneration and/or Emdogain® -: A histologic study

被引:57
作者
Donos, N
Sculean, A
Glavind, L
Reich, E
Karring, T
机构
[1] UCL, Dept Periodontol, Eastman Dent Inst, London WC1X 8LD, England
[2] Univ Aarhus, Royal Dent Coll, Dept Periodontol, DK-8000 Aarhus C, Denmark
[3] Univ Saarland, Dept Periodontol, Saarbrucken, Germany
关键词
bioresorbable membranes; degree III furcation defect; enamel matrix proteins; guided tissue regeneration; new attachment;
D O I
10.1046/j.0303-6979.2003.00429.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background: The use of enamel matrix proteins (EMD) has been recently introduced as a new treatment alternative for periodontal regeneration. However, no histological studies are available investigating the effect of EMD in the treatment of degree III furcation involvements. Objectives: The aim of this study was to evaluate the healing of mandibular degree III furcation involvements histologically following treatment with guided tissue regeneration (GTR), EMD and a combination of EMD and GTR. Material and Methods: Degree III furcation involvements were surgically created at the teeth 36, 37, 46, 47 in three monkeys (Macaca fascicularis). Spontaneous healing of the defects was prevented by placing impression material into the defects. After 6 weeks, full-thickness flaps were elevated at the buccal and lingual aspect of the experimental teeth. Following removal of all granulation tissue from the furcation defects, scaling/root planing and conditioning of the root surfaces with 24% EDTA gel, the defects were treated with one of the following treatment modalities: (i) EMD, (ii) GTR or (iii) a combination of EMD and GTR. The defects serving as control did not receive any treatment, except from complete coverage with coronally displaced flaps. After 5 months of healing, the animals were killed and perfused with 10% buffered formalin for fixation. The experimental teeth with surrounding tissues were dissected free, decalcified in EDTA, dehydrated and embedded in paraffin. 8 mum thick histological sections were cut and stained and subsequently examined under the light microscope. Results: The histological analysis revealed that with GTR or combined EMD and GTR treatment, new attachment formation (new cementum with inserting collagen fibers) had occurred on almost the entire circumference of the furcation and new bone was almost filling the defect in the situations where the membrane was not exposed. The sites treated only with EMD exhibited new attachment and new bone formation to a varying extent, while the control sites presented only limited new attachment and bone formation. Conclusion: The results provided histological evidence suggesting that both GTR and EMD may result in true periodontal regeneration, and suggest that this type of healing might be favored by such treatments in comparison with flap surgery.
引用
收藏
页码:1061 / 1068
页数:8
相关论文
共 50 条
[1]   LYOPHILIZED BONE ALLOGRAFTS IN PERIODONTAL INTRAOSSEOUS DEFECTS [J].
ALTIERE, ET ;
REEVE, CM ;
SHERIDAN, PJ .
JOURNAL OF PERIODONTOLOGY, 1979, 50 (10) :510-519
[2]  
[Anonymous], 1995, J PERIODONTOL
[3]   GTR treatment of degree III furcation defects following application of enamel matrix proteins - An experimental study in dogs [J].
Araujo, MG ;
Lindhe, J .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1998, 25 (06) :524-530
[4]   GTR treatment of degree III furcation defects with 2 different resorbable barriers - An experimental study in dogs [J].
Araujo, MG ;
Berglundh, T ;
Lindhe, J .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1998, 25 (03) :253-259
[5]   On the dynamics of periodontal tissue formation in degree III furcation defects - An experimental study in dogs [J].
Araujo, MG ;
Berglundh, T ;
Lindhe, J .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1997, 24 (10) :738-746
[6]   BIOCERAMIC IMPLANTS IN SURGICAL PERIODONTAL DEFECTS - A COMPARISON STUDY [J].
BARNEY, VC ;
LEVIN, MP ;
ADAMS, DF .
JOURNAL OF PERIODONTOLOGY, 1986, 57 (12) :764-770
[7]   A prospective multi-center study evaluating periodontal regeneration for class II furcation invasions and intrabony defects after treatment with a bioabsorbable barrier membrane: 1-year results [J].
Becker, W ;
Becker, BE ;
Mellonig, J ;
Caffesse, RG ;
Warrer, K ;
Caton, JG ;
Reid, T .
JOURNAL OF PERIODONTOLOGY, 1996, 67 (07) :641-649
[8]   HISTOLOGIC EVALUATION OF NEW ATTACHMENT IN HUMANS - A PRELIMINARY-REPORT [J].
BOWERS, GM ;
GRANET, M ;
STEVENS, M ;
EMERSON, J ;
CORIO, R ;
MELLONIG, J ;
LEWIS, SB ;
PELTZMAN, B ;
ROMBERG, E ;
RISOM, L .
JOURNAL OF PERIODONTOLOGY, 1985, 56 (07) :381-396
[9]   FORMATION OF A PERIODONTAL-LIGAMENT AROUND TITANIUM IMPLANTS [J].
BUSER, D ;
WARRER, K ;
KARRING, T .
JOURNAL OF PERIODONTOLOGY, 1990, 61 (09) :597-601
[10]   FURCATION DEFECTS IN DOGS TREATED BY GUIDED TISSUE REGENERATION (GTR) [J].
CAFFESSE, RG ;
DOMINGUEZ, LE ;
NASJLETI, CE ;
CASTELLI, WA ;
MORRISON, EC ;
SMITH, BA .
JOURNAL OF PERIODONTOLOGY, 1990, 61 (01) :45-50