Lentil seed aquaporins form a hetero-oligomer which is phosphorylated by a Mg2+-dependent and Ca2+-regulated kinase

被引：41

作者：

Harvengt, P

Vlerick, A

Fuks, B

Wattiez, R

Ruysschaert, JM

Homble, F

机构：

[1] Free Univ Brussels, Fac Sci, Lab Physiol Vegetale, B-1050 Brussels, Belgium

[2] Univ Mons, Fac Sci, Biochim Lab, B-7000 Mons, Belgium

[3] Free Univ Brussels, Fac Sci, Chim Phys Macromol Interfaces Lab, B-1050 Brussels, Belgium

来源：

BIOCHEMICAL JOURNAL | 2000年 / 352卷

关键词：

calcium-dependent protein kinase; cross-linking; protein body; membrane; water;

D O I：

10.1042/0264-6021:3520183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In plants, aquaporins regulate the water flow through membranes during growth, development and stress responses. We have isolated two isoforms of the aquaporin family from the protein-storage vacuoles of lentil (Lens culinaris Med.) seeds. Chemical cross-linking experiments showed that both isoforms belong to the same oligomer in the membrane and are phosphorylated by a membrane-bound protein kinase. We assigned the kinase activity to a 52 kDa protein that is magnesium-dependent and calcium-regulated.

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页码：183 / 190

页数：8

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