Determination of ephedra alkaloids by high-performance liquid chromatography

被引：11

作者：

Sheu, SJ

Huang, MH ^{[1
]}

机构：

[1] Natl Taiwan Normal Univ, Dept Chem, Taipei 107, Taiwan

[2] Chia Nan Univ Pharm & Sci, Dept Pharm, Tainan 717, Taiwan

来源：

CHROMATOGRAPHIA | 2001年 / 54卷 / 1-2期

关键词：

column liquid chromatography; ephedrae alkaloids;

D O I：

10.1007/BF02491844

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two simple methods were developed for the simultaneous determination of six alkaloids (ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, methylephedrine and methyl-pseudoephedrine) in Ephedrae Herba by high-performance liquid chromatography. The first method was carried out by using a Cosmosil 5C(18)-MS column with a gradient solvent system consisting of a phosphate buffer and acetonitrile, and detection at 210 nm. The contents of alkaloids in non-pretreated ephedra herb extracts could be determined easily in 50 min. Alternatively, the alkaloids could be determined within 35 minutes by using a Cosmosil 5C18-MS column with an isocratic solvent system of a sodium dodecyl sulfate - acetonitrile solution. The two methods are compared and discussed.

引用

页码：117 / 119

页数：3

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