Protection against influenza virus challenge by topical application of influenza DNA vaccine

被引：66

作者：

Watabe, S

Xin, KQ

Ihata, A

Liu, LJ

Honsho, A

Aoki, I

Hamajima, K

Wahren, B

Okuda, K

机构：

[1] Yokohama City Univ, Sch Med, Dept Bacteriol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] Yokohama City Univ, Sch Med, Dept Internal Med, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[3] Yokohama City Univ, Sch Med, Dept Pathol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[4] Karolinska Inst, Microbiol & Tumorbiol Ctr, Swedish Inst Infect Dis Control, Stockholm, Sweden

来源：

VACCINE | 2001年 / 19卷 / 31期

关键词：

topical application; DNA vaccine; influenza virus;

D O I：

10.1016/S0264-410X(01)00194-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We studied the use of a DNA vaccine expressing the matrix (M) gene of the influenza virus A/PR/8/34. Mice were immunized by painting the DNA vaccine three times on the skin after removal of its keratinocytic layers. Immunization by this method produced M-specific antibodies and cytotoxic T lymphocyte (CTL) response, and acquired resistance against influenza virus challenge. This protection was abrogated by the in vivo injection of anti-CDS or anti-CD4 monoclonal antibody. We further found that simultaneous topical application (t.a.) of GM-CSF expression plasmid (pGM-CSF) or liposomes plus mannan produced stronger immune response competence and enhanced the protective effect against influenza virus challenge. The present study revealed that administering DNA vaccine by topical application can elicit both humoral and cell-mediated immunity (CMI). (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：4434 / 4444

页数：11

共 44 条

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