Nucleus extraction from single mounted tissue sections

被引：11

作者：

Liehr, T

Uwe, C

Erich, G

机构：

[1] Inst Human Genet, D-07743 Jena, Germany

[2] Inst Human Genet, D-91054 Erlangen, Germany

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1999年 / 15卷 / 02期

关键词：

nucleus extraction; paraffin-embedded material; FISH;

D O I：

10.1016/S1050-3862(98)00037-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extraction of nuclei from unmounted archived tissue has become a method widely used for molecular cytogenetic investigations, It is a suitable approach to take advantage of the large series of formalin fixed and subsequently paraffin-embedded material which has been collected in the times before interphase cytogenetic analysis has become possible. We present a new kind of assay for the extraction of nuclei from one single mounted tissue section; the extracted interphase nuclei are suitable very well for a fluorescence in situ hybridization (FISH) approach. The method described has been successfully used for the analysis of the INT2/FGF3-amplicon in 20 samples of oral squamous cell carcinoma. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：65 / 69

页数：5

共 15 条

[1] FEASIBILITY OF INSITU HYBRIDIZATION WITH CHROMOSOME SPECIFIC DNA PROBES ON PARAFFIN WAX EMBEDDED TISSUE [J].