Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry using ionic liquid matrices

被引：113

作者：

Zabet-Moghaddam, M ^{[1
]}

Heinzle, E ^{[1
]}

Tholey, A ^{[1
]}

机构：

[1] Univ Saarland, D-66123 Saarbrucken, Germany

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 02期

关键词：

D O I：

10.1002/rcm.1293

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A major problem hampering the use of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for quantitative measurements is the inhomogeneous distribution of analytes and matrices in solid sample preparations. The use of ionic liquids as matrices for the qualitative and quantitative analysis of low molecular weight compounds like amino acids, sugars and vitamins was investigated. The ionic liquid matrices are composed of equimolar combinations of classical MALDI matrices (sinapinic acid, alpha-cyano-4-hydroxycinnamic acid or 2,5-dihydroxybenzoic acid) with organic bases. These matrix systems allow a homogenous sample preparation with a thin ionic liquid layer having negligible vapour pressure. This leads to a facilitated qualitative and quantitative measurement of the analytes compared with classical solid matrices. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：141 / 148

页数：8

共 40 条

[1] Ionic liquids as matrixes for matrix-assisted laser desorption/ionization mass spectrometry [J].