The role of neurosteroids and nongenomic effects of progestins in the ventral tegmental area in mediating sexual receptivity of rodents

Progesterone (P-4) in the ventromedial hypothalamus (VMH) and ventral tegmental (VTA) is important for facilitation of lordosis; however, P-4's actions in these brain areas are different. Using lordosis in rodents as in vivo experimental models, we have examined the effects progestins exert in the midbrain and hypothalamus. Localization and blocker studies indicate that P4's actions in the VMH require intracellular progestin receptors (PRs) but in the VTA they do not. Progestins that have rapid, membrane effects, and/or are devoid of affinity for PRs, facilitate lordosis when applied to the VTA. Manipulation of GABA and/or GABA(A)/benzodiazepine receptor complexes (GBRs) in the VTA alter lordosis, which suggests that progestins may interact with GBRs to facilitate receptivity by enhancing the function of GABAergic neurons. Interfering with P-4'S metabolism to 5 alpha -pregnan-3 alpha -ol-20-one (3 alpha ,5 alpha -THP), the most effective endogenous positive modulator of GBRs, or the biosynthesis of the neurosteroid 3 alpha ,5 alpha THP in the VTA attenuates female sexual behavior in rodents. Stimulation of mitochondrial benzodiazepine receptors (MBRs), which enhance neurosteroid production, by infusions of a MBR agonist to the VTA enhances lordosis. 3a,5a-THP is increased in the midbrain of mated > proestrous > diestrous rodents. These data suggest that 3 alpha ,5 alpha -THP has a proximate modulatory role on lordosis. In summary, the actions Of P-4 in the VTA are different from those in the VMH that involve PRs. In the VTA, P-4 may facilitate lordosis following metabolism to and/or biosynthesis of 3 alpha ,5 alpha -THP, which may have subsequent actions at GBRs and/or Ill to acutely modulate female sexual behavior in rodents. (C) 2001 Academic Press.