Human Crossveinless-2 is a novel inhibitor of bone morphogenetic proteins

被引：67

作者：

Binnerts, ME ^{[1
]}

Wen, XH ^{[1
]}

Canté-Barrett, K ^{[1
]}

Bright, J ^{[1
]}

Chen, HT ^{[1
]}

Asundi, V ^{[1
]}

Sattari, P ^{[1
]}

Tang, T ^{[1
]}

Boyle, B ^{[1
]}

Funk, W ^{[1
]}

Rupp, F ^{[1
]}

机构：

[1] Nuvelo, Sunnyvale, CA 94085 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 315卷 / 02期

关键词：

D O I：

10.1016/j.bbrc.2004.01.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drosophila Crossveinless-2 (dCV-2) is required for local activation of Mad phosphorylation in the fruit fly wing and has been postulated to be a positive regulator of BMP-mediated signaling. In contrast, the presence of 5 Chordin-like cysteine-rich domains in the CV-2 protein suggests that CV-2 belongs to a family of well-established inhibitors of BMP function that includes Chordin and Sog [Development 127 (2000) 3947]. We have identified a human homolog of Drosophila CV-2 (hCV-2). Here we show that purified recombinant hCV-2 protein inhibits BMP-2 and BMP-4 dependent osteogenic differentiation of W-20-17 cells, as well as BMP dependent chondrogenic differentiation of ATDC5 cells. Interestingly, hCV-2 messenger RNA is expressed at high levels in human primary chondrocytes, whereas expression in primary human osteoblasts is low. These results suggest that hCV-2 may regulate BMP responsiveness of osteoblasts and chondrocytes in vivo. Taken together we have shown that contrary to the function predicted from the fruit fly, Crossveinless-2 is a novel inhibitor of BMP function. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：272 / 280

页数：9

共 36 条

[1] Chordin-like CR domains and the regulation of evolutionarily conserved extracellular signaling systems [J].