Characterization of ileal bacterial microbiota in newly-weaned pigs in response to feeding lincomycin, organic acids or herbal extract

被引:22
作者
Gong, Joshua [1 ,2 ]
Yu, Hai
Liu, Ting
Li, Mei [2 ]
Si, Weiduo
De lange, Cornelis F. M. [2 ]
Dewey, Cate [3 ]
机构
[1] Agr & Agri Food Canada, Food Res Program, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada
[2] Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada
[3] Univ Guelph, Dept Populat Med, Guelph, ON N1G 2W1, Canada
关键词
pigs; ileal microbiota; lincomycin; organic acids; herbal extract; 16S rRNA;
D O I
10.1016/j.livsci.2008.01.001
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The changes of ileal bacterial microbiota in pigs during the first 2 weeks post-weaning in response to feeding lincomycin, organic acids or herbal extract were characterized using 16S rRNA gene-based PCR-denatuxing gradient gel electrophoresis (DGGE) profiling, DNA sequencing, and real-time PCR (QPCR) techniques. Both time post-weaning and the dietary treatments resulted in a shift of the microbiota. composition. While time post-weaning mostly influenced Clostridium group, the feed additives increased the population of Lactobacillus and related lactic acid bacteria by >= 3-fold. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:318 / 322
页数:5
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