Oscillations in the peroxidase-oxidase reaction: A comparison of different peroxidases

被引：31

作者：

Kummer, U ^{[1
]}

Valeur, KR ^{[1
]}

Baier, G ^{[1
]}

Wegmann, K ^{[1
]}

Olsen, LF ^{[1
]}

机构：

[1] ODENSE UNIV,INST BIOCHEM,PHYS BIOCHEM GRP,DK-5230 ODENSE M,DENMARK

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1996年 / 1289卷 / 03期

关键词：

peroxidase-oxidase reaction; oscillation; peroxidase; methylene blue; dichlorophenol;

D O I：

10.1016/0304-4165(95)00176-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nonlinear behavior of the peroxidase-oxidase reaction was studied using structurally different peroxidases. For the first time sustained oscillations with peroxidases other than horseradish peroxidase in a single-enzyme system were observed. All peroxidases that showed significant oxidase activity were able to generate sustained oscillations. When adjusting the overall reaction rate, either of the two modifiers 2,4-dichlorophenol or Methylene blue could be omitted from the reaction. Due to the observation of different enzyme intermediates when using different peroxidases, we conclude that the mechanisms responsible for oscillatory kinetics may vary from one peroxidase to the other.

引用

页码：397 / 403

页数：7

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