TROSY in NMR studies of the structure and function of large biological macromolecules

被引：107

作者：

Fernández, C ^{[1
]}

Wider, G ^{[1
]}

机构：

[1] ETH, Inst Molekularbiol & Biophys, CH-8093 Zurich, Switzerland

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2003年 / 13卷 / 05期

关键词：

D O I：

10.1016/j.sbi.2003.09.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transverse relaxation-optimized spectroscopy (TROSY), in combination with various isotope-labeling techniques, has opened avenues to study biomolecules with molecular masses of up to 1 000 000 Da by solution NMR. Important recent applications of TROSY include the structure determination of membrane proteins in detergent micelles, structural and functional studies of large proteins in both monomeric form and macromolecular complexes, and investigations of intermolecular interactions in large complexes. TROSY improves the measurement of residual dipolar couplings and the detection of scalar couplings across hydrogen bonds - techniques that promise to further enhance the determination of solution structures of large proteins and oligonucleotides.

引用

页码：570 / 580

页数：11

共 65 条

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