Enhancement of biosensor sensitivity in aqueous and organic solvents using a combination of poly(pyrrole-ammonium) and poly(pyrrole-lactobionamide) films as host matrices.

被引:47
作者
Cosnier, S
Lepellec, A
Guidetti, B
Rico-Lattes, I
机构
[1] Univ Grenoble 1, CNRS, UMR 5630, Lab Electrochim Organ & Photochim Redox, F-38041 Grenoble 09, France
[2] Univ Toulouse 3, CNRS, UMR 5623, Lab Interact Mol & React Chim & Photochim, F-31062 Toulouse, France
来源
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1998年 / 449卷 / 1-2期
关键词
glucose oxidase; lactobionamide; polyphenol oxidase; polypyrrole; organic phase biosensor;
D O I
10.1016/S0022-0728(98)00049-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new amphiphilic lactobionamide functionalized by a pyrrole group has been synthesized and electrochemically characterized. This functionalization has allowed the preparation of poly(pyrrole-lactobionamide) films by oxidative electropolymerization in aqueous and organic electrolytes. The electrochemical immobilization of glucose oxidase (GOx) and polyphenol oxidase (PPO) is carried out in polymeric 'bilayer' and 'trilayer' matrices based on poly(pyrrole-ammonium) and poly(pyrrole-lactobionamide) films. The amperometric response of GOx-based biosensors to glucose in phosphate buffer was based on the oxidation at + 0.6 V vs. SCE of the generated H2O2. A marked enhancement of the biosensor sensitivity (+ 60%) was observed for a trilayer matrix illustrating the beneficial effect of the polymerized lactobionamide groups. The amperometric response of PPO-based biosensors to catechol in chloroform was based on the reduction at - 0.25 V vs. SCE of the generated o-quinone. In the same way, the presence of poly(pyrrole-lactobionamide) as a polymeric additive induces a gnat enhancement of the biosensor sensitivity (+ 350%) in anhydrous chloroform. (C) 1998 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:165 / 171
页数:7
相关论文
共 52 条
  • [1] KINETIC-STUDY OF THE INHIBITORY EFFECTS OF METHYL ISOTHIOCYANATE ON A PEROXIDASE-MODIFIED PLATINUM-ELECTRODE IN NONAQUEOUS MEDIA
    ADEYOJU, O
    IWUOHA, EI
    SMYTH, MR
    [J]. ANALYTICAL LETTERS, 1994, 27 (11) : 2071 - 2081
  • [2] REACTIVITIES OF AMPEROMETRIC ORGANIC-PHASE PEROXIDASE-MODIFIED ELECTRODES IN THE PRESENCE AND ABSENCE OF THIOUREA AND ETHYLENETHIOUREA AS INHIBITORS
    ADEYOJU, O
    IWUOHA, EI
    SMYTH, MR
    [J]. ANALYTICA CHIMICA ACTA, 1995, 305 (1-3) : 57 - 64
  • [3] BARTLETT PN, 1993, J ELECTROANAL CHEM, V362, P1
  • [4] Improvement of the analytical characteristics of an enzyme electrode for free and total cholesterol via laponite clay additives
    Besombes, JL
    Cosnier, S
    Labbe, P
    Reverdy, G
    [J]. ANALYTICA CHIMICA ACTA, 1995, 317 (1-3) : 275 - 280
  • [5] Polyphenol oxidase-catechol: An electroenzymatic model system for characterizing the performance of matrices for biosensors
    Besombes, JL
    Cosnier, S
    Labbe, P
    [J]. TALANTA, 1996, 43 (09) : 1615 - 1619
  • [6] ENZYMATIC FLOW-INJECTION ANALYSIS IN NONAQUEOUS MEDIA
    BRACO, L
    DAROS, JA
    DELAGUARDIA, M
    [J]. ANALYTICAL CHEMISTRY, 1992, 64 (02) : 129 - 133
  • [7] Brisset F, 1996, NEW J CHEM, V20, P595
  • [8] NEW ENZYME SENSOR FOR PHENOL DETERMINATION IN NONAQUEOUS AND AQUEOUS-MEDIUM
    CAMPANELLA, L
    SAMMARTINO, MP
    TOMASSETTI, M
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 1992, 7 (1-3) : 383 - 388
  • [9] POLY(AMPHIPHILIC PYRROLE)-PPO ELECTRODES FOR ORGANIC-PHASE ENZYMATIC ASSAY
    COCHEGUERENTE, L
    COSNIER, S
    INNOCENT, C
    [J]. ANALYTICAL LETTERS, 1995, 28 (06) : 1005 - 1016
  • [10] DEVELOPMENT OF AMPEROMETRIC BIOSENSORS BASED ON THE IMMOBILIZATION OF ENZYMES IN POLYMER-FILMS ELECTROGENERATED FROM A SERIES OF AMPHIPHILIC PYRROLE DERIVATIVES
    COCHEGUERENTE, L
    COSNIER, S
    INNOCENT, C
    MAILLEY, P
    [J]. ANALYTICA CHIMICA ACTA, 1995, 311 (01) : 23 - 30